background: the ability to sensitively detect vibrio cholera with pcr-elisa method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. the aim of this research is to evaluate the suitability of a pcr-enzyme-linked immunosorbent assay for sensitive and rapid detection of v. cholera o1. methods: the 398-bp sequence of a gene that code...

The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...

Background: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. Methods: The 398-bp sequence of a gene that cod...

Background: Food adulteration with pork in processed beef products is one of the most serious issues in a food sector in a Muslim-majority country since it is related to religious food ethics regarding the halal products. The goal of this research is to test the suitability of ingredients in beef floss and its Halal by knowing the presence of pork DNA and protein in those products. Methods: Me...

The use of an adequate concentration of Triton X-100 enhanced immunomagnetic separation of Coxiella burnetii from milk. PCR-enzyme-linked immunosorbent assay (PCR-ELISA) could detect coxiellas more sensitively than could conventional PCR. PCR-ELISA is therefore thought to be suitable for the simultaneous assay of a large number of samples. However, the number of cows from which raw milk tested ...

background and objectives : human papillomavirus (hpv) infection is a major risk factor for adenocarcinoma of the cervix. the high-risk types of the virus such as hpv16 and hpv18, which possess the e6 and e7 oncogenes, are responsible for approximately 50% of all cervical cancers. a rapid, sensitive and specific test has been proposed for detection of hpv to improve cervical cancer screening pr...

Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that PCR tests, which considered gold standard. In present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve LOD antigen coronavirus disease 2019 (COVID-19). For recombinant...


 The present study aimed to assess enzyme-linked immunosorbent assay (ELISA) and nested-polymerase chain reaction (n-PCR) methods based on B1 gene for the detection of Toxoplasma (T.) gondii in blood milk local Iraqi goats. SAG3 was also used identify genotyping T. goats aborted women Iraq. A total 240 (80 blood, 80 sera, milk) lactating 30 samples from were included this study. 17 (21.2%...

A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.

A microplate ELISA hybridization assay has been developed for the detection of the IS6110 PCR products of M. tuberculosis from sputum specimens. In this study, its efficacy was evaluated by comparison with agarose gel electrophoresis (AGE) and dot blot hybridization (DBH), with culture results as the 'gold standard'. The assay was used with 190 sputum samples: the PCR results detected by ELISA ...