Key members: Anita Bogdanov Valéria Endrész Szabolcs Urbán [1] Ildikó Lantos Judit Deák Katalin Burián Kamil Önder Ferhan Ayaydin Péter Balázs [2] Dezső P. Virók Founded by: ERA-NET ChlamyTrans grant (D.P. Virok) TKA National Research Fund grant PD 100442 (K. Burián) TÁMOP-4.2.2.A-11/1/KONV-401 2012-0073 (P. Balázs) TÁMOP-4.2.2.A-11-1-KONV-2012-0035 Partners: Institute of Clinical Microbiology,...

BACKGROUND Pregnancy-associated plasma protein-A (PAPP-A) concentrations are increased in the circulation of patients with acute coronary syndromes (ACS) and are associated with future adverse cardiac events. PAPP-A in ACS differs from PAPP-A in pregnancy in that PAPP-A in ACS is not complexed with the proform of eosinophil major basic protein (proMBP). We investigated the effect of antibody se...

BACKGROUND We recently reported that the pregnancy-associated plasma protein A (PAPP-A) form specifically related to acute coronary syndromes (ACS) is not complexed with the proform of eosinophil major basic protein (proMBP). The aim of this study was to develop rapid point-of-care immunoassays for the measurement of the noncomplexed PAPP-A. METHODS We developed immunofluorometric noncompetit...

Pregnancy-associated plasma protein-A (PAPP-A) is a metzincin superfamily metalloproteinase responsible for cleavage of insulin-like growth factor-binding protein-4, thus causing release of bound insulin-like growth factor. PAPP-A is secreted as a dimer of 400 kDa but circulates in pregnancy as a disulfide-bound 500-kDa 2:2 complex with the proform of eosinophil major basic protein (pro-MBP), r...

BACKGROUND Measuring of maternal serum pregnancy-associated plasma protein-A (PAPP-A) in first trimester can be a way for early detection of adverse prenatal outcome due to faulty placenta. OBJECTIVE The aim was to Determination of association between placental thickness in second trimester with low level of PAPP-A in first trimester. MATERIALS AND METHODS In this cohort study, serum PAPP-A...

BACKGROUND First trimester Pregnancy Associated Plasma Protein A (PAPP-A) levels, routinely measured for aneuploidy screening, may predict development of preeclampsia. This study tests the hypothesis that first trimester PAPP-A levels correlate with soluble fms-like tyrosine kinase-1 (sFlt-1) levels, an angiogenic marker associated with preeclampsia, throughout pregnancy. METHODS sFlt-1 level...

Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are proteases that cleave insulin-like growth factor-binding proteins (IGFBPs), resulting in local activation of IGF signaling pathways. Here, we examined PAPP-A and -A2 mRNA and protein levels in placenta and maternal sera from women with pre-eclampsia and compared them with samples from uncomplicated pregnancy. PAPP-A2 but not PAP...

Intense immunostaining for pregnancy-associated plasma protein-A (PAPP-A), a newly characterized metalloproteinase in the insulin-like growth factor system, colocalizes with activated macrophages in human atherosclerotic plaque. To determine macrophage regulation of PAPP-A expression, we developed two models of human macrophages with basal and activated phenotypes. THP-1 cells and peripheral bl...

Activated platelets release a potent inhibitor of factor Xla previously identified as a Kunitz proteinase inhibitor domaincontaining form of the p-amyloid precursor proteins (pAPP). Two carboxy-terminal truncated forms of the pAPP, pAPP751 and pAPP-770. are shown to be the predominant isoforms secreted by platelets. The release of pAPP from platelets is responsible for the higher concentration ...

BACKGROUND Maternal serum concentrations of pregnancy-associated plasma protein-A (PAPP-A, pappalysin-1, EC 3.4.24.79) are used to predict the occurrence of Down syndrome. In pregnancy, PAPP-A primarily circulates as a covalent 2:2 complex with the proform of eosinophil major basic protein (proMBP), which inhibits the proteolytic activity of PAPP-A. At term, however, approximately 1% of PAPP-A ...