Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and con...

background and objectives : the attempts were made to describe the development of a whole cell immobilization of p. pastoris by entrapping the cells in polyacrylamide gel beads. the alcohol oxidase activity of the whole cell pichia pastoris was evaluated in comparison with yeast biomass production. materials and methods : methylotrophic yeast p. pastoris was obtained from collection of standard...

Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...

allopurinol, the xanthine oxidase inhibitor, is the only drug available for the treatment of gout. we examined the xanthine oxidase inhibitory activity of some commercially available flavonoids such asepigallocatechin, acacatechin, myricetin, naringenin, daidzein and glycitein by virtual screening and in-vitro studies. the interacting residues within the complex model and their contact types we...

introduction: oxalate oxidase (ec.1, 2, 3, 4), an acute phase protein of plants, is used widely in agriculture, food industries, medical diagnosis of kidney stones and oxaluria. in this study oxalate oxidase from barley was purified and partially characterized. materials & methods: roots and sprouts of barley cultured in hydroponic states were separated and extracted. ion exchange resins of the...

Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...

onion (allium cepa) contains high levels of flavonoids. although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. fresh filtered juice of onion was prepared and its inhibitory effect on guinea ...

Glucose oxidase is an important enzyme hydrolyzing for its hydrolyzing activity on glucos. It possesses and has a wide board of applications in different industries such as bakery, pharmaceutical, plant pathology and biosensors. In this study, yeast (Saccharomyces cerevisiae) was transformed successfully by the glucose oxidase gene (gox) obtained from Penicillium funicolusum. The secreted gluco...

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...