نتایج جستجو برای: oxacillin screen agar

تعداد نتایج: 102281  

Journal: :iranian journal of pathology 0
hossein koupahi dept. of microbiology, islamic azad university, varamin-pishva branch, varamin, iran sahar honarmand jahromy dept. of microbiology, islamic azad university, varamin-pishva branch, varamin, iran mohammad rahbar dept. of microbiology, iranian reference health laboratory research center, ministry of health and medical education, tehran, iran

background: methicillin resistant staphylococcus aureus (mrsa) has been emerged as a nosocomial and community acquired pathogen worldwide. there are many challenges for laboratory detection of mrsa. the aim of this study was to compare different phenotypic methods with pcr based method as a gold standard for detection of meca gene. methods: a total of 220 clinical isolates of s. aureus which we...

Journal: :Journal of clinical microbiology 2001
J M Swenson P P Williams G Killgore C M O'Hara F C Tenover

Using a set of 55 Staphylococcus aureus challenge organisms, we evaluated six routine methods (broth microdilution, disk diffusion, oxacillin agar screen, MicroScan conventional panels, MicroScan rapid panels, and Vitek cards) currently used in many clinical laboratories and two new rapid methods, Velogene and the MRSA-Screen, that require less than a day to determine the susceptibility of S. a...

Journal: :Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases 2004
Haruhiko Taguchi Takamasa Kaneko Masanobu Onozaki Ryoichi Kubo Shigeru Kamiya

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious nosocomial and community-acquired infections, and accurate methods to detect such strains are needed. We tested the susceptibility of 3 kinds of MRSA isolation medium, MRSA Screen Agar, Oxacillin Resistance Screening Agar and CHROMagar MRSA. Both sensitivity and specificity of CHROMagar MRSA we...

Journal: :Journal of clinical microbiology 1999
M Cavassini A Wenger K Jaton D S Blanc J Bille

The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of the mecA gene for the detection of methicillin resistance in Staphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) ...

Journal: :Biomedical journal 2014
Arunava Kali Selvaraj Stephen Sivaraman Umadevi

Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred ...

2006
Mohammad Rahbar Mahmood Yaghoobi Ali Fattahi

Objective: The aim of this study was to compare four different laboratory methods for detection of methicillin resistant Staphylococcus aureus (MRSA). Design: The clinical specimens including urine, blood, wound and tracheal tube aspirates were processed for isolation of S .aureus. Setting: The samples were obtained from patients admitted in Milad hospital in Tehran,Iran. Subjects: 95 strains o...

Journal: :Journal of clinical microbiology 1999
P Kohner J Uhl C Kolbert D Persing F Cockerill

Ninety-nine clinical staphylococcal isolates (58 coagulase-negative Staphylococcus spp. [CoNS] and 41 Staphylococcus aureus isolates) were evaluated for susceptibility to oxacillin. The following susceptibility testing methods, media, and incubation conditions were studied: agar dilution by using Mueller-Hinton (MH) medium (Difco) supplemented with either 0, 2, or 4% NaCl and incubation at 30 o...

Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates. Materials and Method...

Journal: :Journal of clinical microbiology 2004
John H Lee Jae-Myung Jeong Yong-Ho Park Sung-Sun Choi Yong-Hwan Kim Joon-Seok Chae Jin-San Moon Hyun Park Shin Kim Seong-Kug Eo

Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analys...

Journal: :Journal of clinical microbiology 1999
A van Griethuysen M Pouw N van Leeuwen M Heck P Willemse A Buiting J Kluytmans

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a in Staphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methi...

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