background: relapsing fever caused by borrelia persica , is an acute tick-borne disease which is transmitted by soft ticks of ornithodoros tholozani to human.     methods: value of pcr and xenodiagnosis for detection of b. persica in o. tholozani ticks was compared. sixty-four bor­relia -free ticks were fed on infected guinea pigs and used for the experiments. for xenodiagnosis, a group of 32 t...

objective: relapsing fever caused by borrelia persica is an acute tick-borne disease which is transmitted by soft ticks of ornithodoros tholozani to human. the disease is reported from middle east and many regions of iran. detection of infection is problematic since the suspected infected ticks should be fed on animal hosts such as guinea pigs and subsequently after 7-14 days, the animal blood ...

introduction: spirochetes of borrelia can be visualized directly in infected ticks by dark-field microscopy. inoculation of in phosphate buffered saline (pbs) suspension of ground argasid soft ticks to susceptible animals or allowing the ticks to feed on the same species followed by microscopic examination of the animals’ blood have also been practiced. with the advent of molecular methods and ...

BACKGROUND Relapsing fever caused by Borrelia persica, is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human. METHODS Value of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Borrelia-free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in...

T relapsing fever (TBRF) is caused by Borrelia species and is transmitted to humans by Ornithodoros soft ticks. Worldwide, a dozen Borrelia species are known to cause this disease (1). In Israel, TBRF is considered to be caused by Borrelia persica and transmitted by the cave tick Ornithodoros tholozani (1). This tick and TBRF are distributed through Central Asia (2) and the Middle East (1). Oth...

Background: Relapsing fever caused by Borrelia persica, is an acute tick-borne disease which is transmitted by soft ticks of Ornithodoros tholozani to human. Methods: Value of PCR and xenodiagnosis for detection of B. persica in O. tholozani ticks was compared. Sixty-four Borrelia-free ticks were fed on infected guinea pigs and used for the experiments. For xenodiagnosis, a group of 32 ticks in...

The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, ...

Introduction: Spirochetes of Borrelia can be visualized directly in infected ticks by dark-field microscopy. Inoculation of in phosphate buffered saline (PBS) suspension of ground Argasid soft ticks to susceptible animals or allowing the ticks to feed on the same species followed by microscopic examination of the animals’ blood have also been practiced. With the advent of molecular method...