PURPOSE The extracellular matrix glycoprotein opticin is a small leucine-rich repeat proteoglycan/protein family member that was discovered associated with vitreous humor collagen fibrils. Opticin is present throughout the vitreous, but is particularly concentrated at the internal limiting lamina, where it colocalizes with type XVIII collagen. The present study investigated whether opticin inte...

AIM To localise the recently discovered glycoprotein opticin in the adult human eye. METHODS Polyclonal rabbit antisera were raised against two different opticin peptides. Isolated human vitreous collagen fibrils were extracted with 8 M urea and the extract analysed by SDS-PAGE and western blotting. Paraffin embedded sections from two normal eyes were subjected to immunohistochemical analysis...

Opticin is an extracellular matrix glycoprotein that we identified associated with the collagen network of the vitreous humor of the eye. Recently, we discovered that opticin possesses anti-angiogenic activity using a murine oxygen-induced retinopathy model: here, we investigate the underlying mechanism. Using an ex vivo chick chorioallantoic membrane assay, we show that opticin inhibits angiog...

PURPOSE Opticin is a small leucine-rich repeat proteoglycan that is abundant in several ocular tissues, including the vitreous. Like other proteoglycans, opticin may have the ability to bind and regulate the release of growth factors. Previous work has shown that isoforms of growth hormone (GH) are present in the embryonic retina, where they may act as a growth factor. The current study was con...

objective: the lrr (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, dna repair and rna processing. opticin is a member of this family. takanosu et al (2001) detected messenger rna expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization. in this study, exp...

background: opticin, as a member of small leucine rich proteoglycans (slrps), mainly expressed in human eyes. it is also expressed in human brain, ligament, liver and skin, but at lower levels than the eyes. in the present study, expression of opticin was studied in a variety of human cancer cell lines. materials and methods: in this basic study, human cancer cell lines including, a-172 (brain ...

PURPOSE To analyze the protein profile of human vitreous of untreated patients with retinal vein occlusion (RVO). METHODS Sixty-eight vitreous humor (VH) samples (44 from patients with treatment naïve RVO, 24 controls with idiopathic floaters) were analyzed in this clinical-experimental study using capillary electrophoresis coupled to mass spectrometer and tandem mass spectrometry. To define ...