background: the objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and atp content of oocytes. methods: the oocytes at germinal vesicle (gv) and metaphase ii (mii) stages were recovered from 6-8 week old nmri strain female mice. the oocytes were divided into vitrified and non-vitrified groups. vitrification was done b...

background: oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. the present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by cryotop method. method: a total of 200 germinal vesicle (...

background: despite the large number of papers published on the efficiency of different exogenous gonadotropins, no confirmed protocol exists. therefore, the aim of the present study was to compare the efficacy of 4 exogenous gonadotropins in ivf/icsi cycles. methods: this study, performed from january 2014 to may 2014, recruited 160 women referred to ghadir mother and child hospital and dena h...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...

background: in vitro maturation (ivm) is a promising treatment option for certain infertile women. nowadays, with the aid of polscope, it has become possible to evaluate zona pellucida (zp) characteristics as a parameter of oocyte quality. moreover, quality of oocytes can be influenced by many factors, such as patient’s age. the polscope system is a non-invasive technique to assess birefringent...

background: the aim of this study was to investigate the effects of two vitrification protocols on mouse metaphase stage ii (mii) oocytes and evaluate their effects on the expression of heat shock protein a1a (hsp a1a) and mnsod genes. materials and methods: groups of approximately 15 oocytes without cumulus complexes were collected and vitrified with 10% (v/v) ethylene glycol (eg) + 10% (v/v) ...

Background and aims: Various culture media along with some supplements and enrichment materials have been suggested for in vitro maturation. This study aims to investigate the effect of retinoic acid on nuclear maturation of immature mouse oocytes in vitro. Materials and methods: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units ...

Objective:In vitro maturation is a highly effective method for achieving mature  oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. MATERIALS AND METHODS: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of...