objective: to evaluate the effect of cytoplasm transfer from mature oocytes to germinal vesicle(gv)s on promoting the maturation of cytoplasm of gv at the mrna level. materials and methods: sixty six in vitro fertilization (ivf) operations between june 2012 and november 2013 were included in this study. totally 120 gvs were obtained. normal gvs were categorized into 3 groups (n = 40) randomly: ...

BACKGROUND The use of gonadotrophin-releasing hormone (GnRH) agonist for triggering final oocyte maturation and ovulation can reduce ovarian hyperstimulation syndrome (OHSS) in high-risk patients. LH levels post-trigger with GnRH agonist might be correlated with oocyte yield and maturity. Our aim was to evaluate the relationship between serum LH level at 12-h post-trigger and oocyte yield, matu...

eurasian perch, perca fluviatilis l., is one of the most important species in european freshwater aquaculture and probably one of most important of cold freshwater species in nearest future. the aim of this study was to test the ovaprim in artificial reproduction in eurasian perch in two temperatures (12 and 14°c) with using new oocyte maturation stage system. the results obtained in present st...

Background: Recent animal studies demonstrated that regulating the microRNA (miRNA) in granulosa cells (GCs) modulates the meiotic competence of oocytes. However, the difference in expression profiles of miRNAs in human GCs according to the maturity of the oocyte still remains to be elucidated. Objective: This observational study investigated whether the miRNA profile of human GCs differs accor...

OBJECTIVE To evaluate pregnancy outcomes and the incidence of ovarian hyperstimulation syndrome (OHSS) using a sliding scale hCG protocol to trigger oocyte maturity and establish a threshold level of serum b-hCG associated with optimal oocyte maturity. DESIGN Retrospective cohort. SETTING Academic medical center. PATIENTS Fresh IVF cycles from 9/2004-12/2011. INTERVENTION 10,427 fresh I...

Walleye pollock, Theragra chalcogramma, is the most commercially important species in the eastern Bering Sea. Estimates of maturity are critical for setting an appropriate harvest rate for pollock spawning biomass, and correct classification of ovaries into a maturity condition is necessary for accurate estimation of maturity. Data on length, weight, and ovary weight and condition were collecte...

OBJECTIVE During stimulated IVF cycles, up to 15% of oocytes are recovered as immature. The purpose of this study was to investigate the trend of oocyte maturity in repeated ovarian stimulation for IVF. METHODS One hundred forty-eight patients were selected who underwent two consecutive IVF cycles using same stimulation protocol during 2008 to 2010. Ovarian stimulation was performed with FSH ...

Objective The success rates of assisted reproduction technology (ART) could be developed with the improvement of ovarian stimulation protocols as well as the optimization of final oocyte maturation. The goal of this study was to compare oocyte maturation, fertilization and pregnancy rates among women with concomitant FSH administration at the time of hCG trigger and the hCG trigger alone. Mater...

in the present study histological studies was conducted after the female abramis brama orientalis was exposed to eight treatment phases in which lhrha2 (3 μg/kg), metoclopramide (5 mg/kg), and chlorpromazine (10 mg/kg) were inoculated. the treatments were characterized by combined and solitary injections, control group (o injection), and experimental group (saline). the results showed that all ...