Oligosaccharides possess fascinating functions that are applicable in a variety of fields, such as agriculture. However, the selective synthesis oligosaccharides, especially chitin-oligosaccharides, has remained challenge. Chitin-oligosaccharides activate plant immune system, enabling crops to withstand pathogens without harmful agrichemicals. Here, we demonstrate conversion chitin chitin-oligo...

background: necrotizing enterocolitis (nec) is one of the most destructive diseases associated with conditions of neonatal prematurity. supplementation with enteral prebiotics may reduce the incidence of nec, especially in infants who fed exclusively with breast-milk. therefore, we compared the efficacy and safety of enteral supplementation of a prebiotic mixture (short chain galacto-oligosacch...

Comparative study of the oligosaccharide profiles of individual human milk revealed the presence of three different patterns. Four oligosaccharides containing the Fucalpha1-2Gal group were missing in the milk of non-secretor, and three oligosaccharides containing the Fucalpha1-4GlcNAc group were missing in the milk of Lewis negative individuals. Disappearance of some major oligosaccharides in t...

• AOS and GOS decreased the E. coli growth by different concentrations. AOS-Ampicillin exerted 2-fold decrease in MIC level of AMP against . reduced adherence to HT-29 cells. The combination further increased AOS’ anti-adhesive properties. AOS, COS or significantly hampered -induced IL-8 release HT-29. mounting antibiotic resistance emphasizes an urgent need for alternatives. Recent investigati...

s: Several oligosaccharides such as glycosylsucrose, fructo-oligosaccharides, maltooligosaccharides, isomalto-oligosaccharides (branched-oligosaccharides), galacto-oligosaccharides, xylo-oligosaccharides, isomaltulose (palatinose), and lactosucrose have been produced on an industrial scale. Recent developments in industrial enzymology have made possible a series of new starch oligosaccharides s...

Abstract Polygonatum sibiricum, famous for its physiological activities, is a popular Chinese food and also traditional herb containing many carbohydrates as the main component. However, except most reported polysaccharides, other detailed compositions of were still not clear. To verify controversial existence starch investigate components especially about oligosaccharides, we used iodine-potas...

Glc3Man9(GlcNAc)2-pyrophosphoryl-dolichol is the major lipid-linked oligosaccharide (LLO) produced by Madin-Darby canine kidney cells in culture. However, when these cells are incubated in the presence of millimolar concentrations of mannosamine and labeled with [2-3H]mannose, they accumulate various LLO that have smaller-sized oligosaccharides with unusual structures and the Glc3Man9(GlcNAc)2-...

The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation based on tandem mass spectrometry. Ions commonly are selected and subjected to collision-induced dissociation (CID) to obtain structural information. Unfortunately, N-linked oligosaccharides are relatively large compounds and are not readi...

This study synthesized resveratrol oligosaccharides (gluco oligosaccharides) by two-step enzymatic glycosylation, using glucosyltransferase from Phytolacca americana and cyclodextrin glucanotransferase. Although hardly crosses the blood–brain barrier (BBB) in mouse brain, incorporated into brain tissue crossed BBB. Our indicated that modified with might have gained a BBB-crossing ability. Furth...

A new anion dopant for oligosaccharides is developed for use in matrix-assisted laser desorption/ionization mass spectrometry. Two types of sulfate-attached quasimolecular ions are formed in the negative ion mode when neutral oligosaccharides are doped with dilute H2SO4 solutions. Under mild conditions, i.e., low H2SO4 concentration (approximately 10(-3) M) and threshold laser fluence, a sulfat...