conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

in this study, the bacterial diversity in the activated sludge system of a full-scale municipalwastewater treatment plant in dubai was monitored over a period of one year using ribosomal rna (rrna)targeted oligonucleotide probes for a defined phylogenetic group of bacteria by the fluorescence in situhybridization (fish) technique. the largest fraction of the bacterial community in the sludge sa...

in this study, the bacterial diversity in the activated sludge system of a full-scale municipalwastewater treatment plant in dubai was monitored over a period of one year using ribosomal rna (rrna)targeted oligonucleotide probes for a defined phylogenetic group of bacteria by the fluorescence in situhybridization (fish) technique. the largest fraction of the bacterial community in the sludge sa...

To examine the utility and performance of 50mer oligonucleotide (oligonucleotide probe) microarrays, gene-specific oligonucleotide probes were spotted along with PCR probes onto glass microarrays and the performance of each probe type was evaluated. The specificity of oligonucleotide probes was studied using target RNAs that shared various degrees of sequence similarity. Sensitivity was defined...

Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or gene families. Only recently has there been ...

Microarrays with oligonucleotides of different lengths were used to monitor gene expression at a whole-genome level. To determine what length of oligonucleotide is a better alternative to PCR-generated probes, the performance of oligonucleotide probes was systematically compared to that of their PCR-generated counterparts for 96 genes from Shewanella oneidensis MR-1 in terms of overall signal i...