objective(s):  although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. in this study, hydroxyapatite nanoparticles (nps) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (dope)-modified hydroxyapatite nps was coated with antisense oligonucleotide of e6 mrna...

Abstract Herein, we describe the construction of a reduction-responsive oligonucleotide by post-modification an with diazo compound bearing 4-nitrobenzyl group as cleavable moiety. High-performance liquid chromatography and mass spectrometry were used to reveal introduction 5?-phosphate oligonucleotide, subsequent reduction-triggered recovery original oligonucleotide. The protocol for preparati...

background: malignant melanoma is a highly metastatic cutaneous cancer and typically refractory to chemotherapy. deregulated apoptosis has been identified as a major cause of cancer drug resistance, and upregulated expression of the inhibitor of apoptosis protein melanom, an inhibitor of apoptosis (ml-iap) is frequent in melanoma. methods: based on the conclusion that ml-iap expression contribu...

background: bacterial dna has immunostimulatory effects on different types of immune cells such as dendritic cells (dcs). application of dcs as a cellular adjuvant represents a promising approach in the immunotherapy of infectious disease and cancers. objectives: to investigate the effect of tumor antigen pulsed dcs in the presence of cpg-1826 in treatment of a murine model of cancer. methods: ...

Background: Malignant melanoma is a highly metastatic cutaneous cancer and typically refractory to chemotherapy. Deregulated apoptosis has been identified as a major cause of cancer drug resistance, and upregulated expression of the inhibitor of apoptosis protein melanom, an inhibitor of apoptosis (ML-IAP) is frequent in melanoma. Methods: Based on the conclusion that ML-IAP expression contribu...

Progress with antisense oligonucleotide therapies opens a path for future development

conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

Can the green innovations introduced in peptide synthesis be transferred to oligonucleotide manufacturing? A critical analysis disclose potential of TIDES.

Gene therapy is in its development stage as a novel method for cancer treatment. Liposomes look promising as gene delivery vectors; however, investigations have shown that these vesicles are not doing well in some cases. It was decided here to investigate the possibility of augmentation of liposomal gene delivery by chemical penetration enhancers.Cationic liposome containing antisense oligonucl...

The not-for profit foundation n-Lorem seeks to provide experimental antisense oligonucleotide treatments patients with ultra-rare disease free for their entire lifetime.