BACKGROUND/AIM Outer inflammatory protein A (OipA) is an important adhesin of Helicobacter pylori. Our goal was to assess the role of OipA in protection of C57BL/6 mice against H. pylori. MATERIALS AND METHODS C57BL/6 mice were mucosally immunized with recombinant OipA protein, OipA + propolis, propolis, and phosphate-buffered saline. After vaccination, anti-OipA IgA was measured. Mice were c...

BACKGROUND There are increasing studies examining the relationship between the status of H. pylori oipA gene and peptic ulcer disease (PUD) and gastric cancer (GC) but the results turn out to be controversial. We attempted to clarify whether oipA gene status is linked with PUD and/or GC risks. METHODS A systematically literature search was performed through four electronic databases. Accordin...

RANTES, a CC chemokine, plays an important role in the inflammatory response associated with Helicobacter pylori infection. However, the mechanism by which H. pylori induces RANTES expression in the gastric mucosa is unknown. We cocultured gastric epithelial cells with wild-type H. pylori, isogenic oipA mutants, cag pathogenicity island (PAI) mutants, or double knockout mutants. Reverse transcr...

The regulation of Helicobacter pylori induced interleukin (IL)-6 in the gastric epithelium remains unclear. Primary gastric epithelial cells and MKN28 cells were cocultured with H. pylori and its isogenic cag pathogenicity island (PAI) mutant and/or oipA mutants. H. pylori infection-induced IL-6 mRNA expression and IL-6 protein production, which was further enhanced by the cag PAI and OipA. Luc...

Đặt vấn đề: Nhiễm vi khuẩn Helicobacter pylori (H. pylori) liên quan chặt chẽ đến các bệnh lý dạ dày như: VDDM, LDD và UTDD. CagA VacA là hai yếu tố độc lực của H. đã được nghiên cứu nhiều ở Việt Nam. Một số khác (oipA, dupA, cag-PAI) chưa tại Mục tiêu: Nghiên tìm hiểu vai trò OipA nhân UTDD Đối tượng & phương pháp: cắt ngang trên 623 nhiễm trong đó có: (n =154), = 129), LTT 161 ) VDDM =179...

AIM The purpose of this investigation was to determine the oipA and dupA genes of Helicobacter pylori isolates from west of Iran; Chaharmahalo Bakhtiyari region and find their relationship with the severity of the gastroduodenal diseases. BACKGROUND Helicobacter pylori is an organism responsible for many gastroduodenal diseases. Many studies suggest that genetic diversity in H . pylori virule...

Helicobacter pylori infection is now accepted as the major cause of severe gastritis-associated diseases, including peptic ulcer and gastric cancer. In the current study, we decided to determine the dupA and OipA genotypes in H. pylori strains isolated from patients with gastrointestinal disorders and to evaluate whether these genes are associated with the severity of the gastroduodenal disease...

Background: Outer inflammatory protein A (OipA) is one of the important adhesins of H. pylori and a valuable candidate for vaccine development. Its gene is under "on-off" switch status which correlates with OipA protein expression. Objectives: We aimed to obtain a recombinant OipA clone (with "on" status) from an Iranian clinical isolate. Materials and Methods: A clinical H. pylori-isolate demo...

Helicobacter pylori is the strongest known risk factor for gastric adenocarcinoma, and strains that possess the cag secretion system, which translocates the bacterial effector CagA into host cells, augment cancer risk. H. pylori strains that express the vacuolating cytotoxin or the outer membrane protein OipA are similarly associated with severe pathologic outcomes. We previously reported that ...

The most common Helicobacter pylori genotype among 37 U.S. children was cagA positive, vacA s1m1, and oipA "on" (n = 17, 45.9%), followed by cagA negative, vacA s2m2, and oipA "off" (n = 8, 21.6%), similar to the pattern in adults. cagA positivity was more common in blacks than in whites (i.e., 100% versus 56.5%, P = 0.032).