Tetramethylammonium hydroxide (TMAH) thermochemolysis has been utilized by the Curiosity rover’s Sample Analysis at Mars (SAM) instrument and will be ExoMars 2022′s Organic Analyzer (MOMA) instrument. TMAH is one of main techniques that enables detection bioindicators when it combined with pyrolysis-gas chromatography mass spectrometry (Py-GC/MS) surface Mars. This study identifies products tar...

DOI: 10.1002/jssc.202001081 The cover picture shows a new HPLC strategy for quantitative analysis of multiple components via single marker (QAMS) to determine nine nucleosides (cytidine, uridine, 2'‒deoxyuridine, inosine, guanosine, 2'‒deoxyguanosine, thymidine, adenosine and 2'‒deoxyadenosine) in Rhizoma Paridis the first time. results methodology validation showed that there was no significan...

An efficient and facile strategy has been developed for bromination of nucleosides using sodium monobromoisocyanurate (SMBI). Our methodology demonstrates bromination at the C-5 position of pyrimidine nucleosides and the C-8 position of purine nucleosides. Unprotected and also several protected nucleosides were brominated in moderate to high yields following this procedure.

Modified urinary nucleosides are potentially invaluable in cancer diagnosis, as they reflect altered RNA turnovers. High-performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, tandem mass spectrometry, MS(n) analysis and accurate mass measurements in order to identify pyrimidine nucleosides purified from urine. Potential nucleosides were assessed by their eviden...

detritylation of 5'-dimethoxytrityl nucleosides have been quantitatively achieved in minutes at room temperature under aprotic neutral conditions by using stannous chloride. of additional practical consideration and in contrast to protic acids, no depurination was observed with this reagent.

Detritylation of 5'-dimethoxytrityl nucleosides have been quantitatively achieved in minutes at room temperature under aprotic neutral conditions by using stannous chloride. Of additional practical consideration and in contrast to protic acids, no depurination was observed with this reagent.

Urinary nucleosides are associated with many types of cancer. In this study, six targeted urinary nucleosides, namely adenosine, cytidine, 3-methylcytidine, 1-methyladenosine, inosine, and 2-deoxyguanosine, were chosen to evaluate their role as biomarkers of four different types of cancer: lung cancer, gastric cancer, colon cancer, and breast cancer. Urine samples were purified using solid-phas...

A possibility of the one-pot synthesis of purine and pyrimidine nucleosides employing pure recombinant ribokinase, phosphopentomutase and nucleoside phosphorylases in a caskade transformation of D-pentoses into nucleosides is demonstrated. Preliminary results of this study point to reliability to develop practical methods for the preparation of a number of biologically important nucleosides.

Fifteen purine nucleosides and their O-acetylated ester derivatives were examined for in vitro antitrypanosomal activity against the LAB 110 EATRO isolate of Trypanosoma brucei brucei and two clinical isolates of Trypanosoma brucei rhodesiense. Initial comparisons of activity were made for the LAB 110 EATRO isolate. Three nucleoside analogs exhibited no significant activity (50% inhibitory conc...

nucleotide synthesis. Individual nucleosides and nucleobases also serve a variety of specialized functions. Various structural analogues of nucleoside and nucleobase are cytotoxic and have found expanding therapeutic use as antineoplastic agents. Nucleosides and nucleobases are hydrophilic and require specific transporters for permeation through the cell membrane. Several mammalian nucleoside t...