conclusions the pe gene was successfully cloned and confirmed by sequencing. finally, pe was obtained with high concentration. due to high homology and similarity among the pe gene from nthi atcc 49766 and other nthi strains in genbank, we believe that the protein is a universal antigen to be used as a vaccine design candidate and further studies to evaluate its immunogenicity is underway. mate...

There is a particular need in protein analysis and purification for specific, functional, and generic methods of protein immobilization on solid supports. Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, which shows at least 1 order of magnitude stronger binding to Ni-NTA-modified surfaces than a conventional single...

Ni2+-functionalized porous ceramic/agarose composite beads (Ni-NTA Cerose) can be used as carrier materials to immobilize enzymes harboring a metal affinity tag. Here, 6×His-tag fusion alcohol dehydrogenase Mu-S5 and glucose from Bacillus megaterium (BmGDH) were co-immobilized on Ni-NTA Cerose construct packed bed reactor (PBR) for the continuous synthesis of chiral intermediate (S)-(4-chloroph...

The design of proteins represents a significant challenge to modern-day structural biology. A major obstacle here is the specification of well-packed hydrophobic cores to drive the folding and stabilization of the target. Computational approaches have been used to alleviate this by testing alternate sequences prior to the production and characterization of a few proteins. Here we present the ex...

The success of recombinant protein expression/purification in Escherichia coli depends on a high-fidelity system rendering purified proteins free of confounding contaminants such as endotoxin. Here we report on the expression and purification of a cryptic plasminogen-derived domain, kringle 5, which was previously reported to specifically inhibit endothelial cell growth and, therefore, angiogen...

15 2DTA 2NTA NTA p MITL k U k DTA Figure 5: Relationship between various classes Theorem 5.2 The membership problem for 2NTA is solvable in PSPACE. Proof. Consider a 2NTA A, and let k be the magnitude of the largest constant appearing in a clock constraint in the transitions of A. Given a timed word w = (;) of length n, deene R w to be the set comprising of i + c and (i + j + c)=2 for 1 i; j n ...

The interaction of Regulator of G protein Signaling 4 (RGS4) with the rat mu opioid receptor (MOR)/G protein complex was investigated. Solubilized MOR from rat brain membranes was immunoprecipitated in the presence of RGS4 with antibodies against the N-terminus of MOR (anti-MOR10-70 ). Activation of MOR with [D-Ala(2) , N-Me-Phe(4) , Gly(5) -ol] enkephalin (DAMGO) during immunoprecipitation cau...

BACKGROUND Adhesion protein E (PE) of Haemophilus influenzae is a 16 - 18 kDa protein with 160 amino acids which causes adhesion to epithelial cells and acts as a major factor in pathogenesis. OBJECTIVES In this study, we performed cloning, expression and purification of PE as a candidate antigen for vaccine design upon further study. MATERIALS AND METHODS At first, the pe gene of NTHi ATCC...