conclusions overall, results of present study were consistent with the data reported earlier base on 5’ ntr in iranian isolates and revealed genotype ir2 is major genotype in iranian hgv-positive patient. it means there are higher similarities between iranian and europe-usa in hgv ns3 gene. results nucleotide blast results emphasized that most similar sequences to ir ns3 sequences were those fr...

conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...

background and aims: dna constructs containing hcv antigens have become one of the vaccine candidates for induction of anti-hcv cellular and humoral immunity. in this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of ns3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...

Background: The yellow mealworm beetle, Tenebrio molitor, is a promising alternative protein source for animal and human nutrition its farming involves relatively low environmental costs. For these reasons, industrial scale production started this century. However, to optimize breed sustainable new T. molitor lines, t...

Background: A recent bioinformatics technique involves changing nucleotide sequences into 2D speckles. This produces speckles called GB-speckles (Gene Based speckles). All classical strategies of speckle-optics, namely speckle-interferometry, subtraction speckle-images as well speckle-correlometry have been inferred for processing GB-speckles. indicates the considera...

Background: Duchenne muscular dystrophy (DMD) and Becker (BMD) are allelic disorders caused by mutations in the DMD gene. The full mutation spectrum of DMD gene Indonesian patients is currently unknown. Mutation-specific therapies being developed, such as exon skipping or stop codon read-through therapy. This study was conducted with aim ...

Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain activate neuroinflammatory release cytokines. This study analyzed effect of co-culture neuron cells with P. gingivalis coated anti-P. antibodies against cytokines ...

introduction: long term complications of hepatitis c virus (hcv) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. therefore, development of effective, safe and affordable proph...