Background: Human peripheral blood NK cells constitutively express CD56 and CD16 antigens. Peripheral blood NK cells seem to be strongly related with decidual NK cells, and may reflect the decidual NK cell functional status. There are varied reports concerning the relationship between NK cell cytotoxicity in women with recurrent spontaneous abortion. Objective: To study NK activity in women wit...

background: human peripheral blood nk cells constitutively express cd56 and cd16 antigens. peripheral blood nk cells seem to be strongly related with decidual nk cells, and may reflect the decidual nk cell functional status. there are varied reports concerning the relationship between nk cell cytotoxicity in women with recurrent spontaneous abortion. objective: to study nk activity in women wit...

Natural killer (NK) cells play a crucial role in regulating immune functions. Few studies have characterized canine NK cells. We previously demonstrated that canine peripheral blood lymphocytes (PBLs) with a low surface CD5 density (CD5lo) are considered a critical NK population. Natural killer cells in most mammals do not express T-cell markers, but canine CD5lo cells express surface molecules...

background: hla-g is a nonclassical hla class i molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including nk and t cells. objective: to evaluate the effect of hla-g downregulation on nk cell cytotoxicity in tumor cell lines. methods: the expression level of hla-g was measured by real-time pcr and flowcytometry after transfec...

Background: HLA-G is a nonclassical HLA class I molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including NK and T cells. Objective: To evaluate the effect of HLA-G downregulation on NK cell cytotoxicity in tumor cell lines. Methods: The expression level of HLA-G was measured by real-time PCR and flowcytometry after transfec...

The effect of natural killer (NK) cells and lymphokine activated killer ( LAK) cells was studied on a group of human melanoma cell lines. Peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhI L-2 for producing LAK cells. The cytotoxicity of effector cells was quantified using a 4 hour SI determining the density of antigen expression on tumor...

hematopoietic cord blood (cb) stem cell transplantation has more advantages to other cell sources because of lower graft versus host disease (gvhd). interleukin-15(il-15) is an immunoregulatory cytokine, known to enhance cytolytic function of cord natural killer (nk) cells. the aim of this study was to investigate the effect of il-15 on nk cytotoxicity simultaneously in different cell death sta...

the effect of natural killer (nk) cells and lymphokine activated killer ( lak) cells was studied on a group of human melanoma cell lines. peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhi l-2 for producing lak cells. the cytotoxicity of effector cells was quantified using a 4 hour si determining the density of antigen expression on tumor cel...

Background:  Natural Killer cells express killer inhibitory receptors specific for HLA-class I molecules.  These receptors could induce signals that determine NK cells ability to mediate cytotoxicity.  Purified soluble form of HLA class I molecules (sHLA) could bind to NK cell receptors and down-regulate the NK killer function. Objective:  To evaluate the influence of sHLA and two monoclonal an...