نتایج جستجو برای: nhx1

تعداد نتایج: 81  

Journal: :The Biochemical journal 2000
C P Darley O C van Wuytswinkel K van der Woude W H Mager A H de Boer

Sodium at high millimolar levels in the cytoplasm is toxic to plant and yeast cells. Sequestration of Na(+) ions into the vacuole is one mechanism to confer Na(+)-tolerance on these organisms. In the present study we provide direct evidence that the Arabidopsis thaliana At-NHX1 gene and the yeast NHX1 gene encode low-affinity electroneutral Na(+)/H(+) exchangers. We took advantage of the abilit...

Journal: :The Plant cell 2011
Elias Bassil Hiromi Tajima Yin-Chih Liang Masa-Aki Ohto Koichiro Ushijima Ryohei Nakano Tomoya Esumi Ardian Coku Mark Belmonte Eduardo Blumwald

Intracellular Na(+)/H(+) (NHX) antiporters have important roles in cellular pH and Na(+), K(+) homeostasis. The six Arabidopsis thaliana intracellular NHX members are divided into two groups, endosomal (NHX5 and NHX6) and vacuolar (NHX1 to NHX4). Of the vacuolar members, NHX1 has been characterized functionally, but the remaining members have largely unknown roles. Using reverse genetics, we sh...

Journal: :The Journal of biological chemistry 2011
Laura M Kallay Christopher L Brett Deepali N Tukaye Megan A Wemmer Anthony Chyou Greg Odorizzi Rajini Rao

The multivesicular body (MVB) is an endosomal intermediate containing intralumenal vesicles destined for membrane protein degradation in the lysosome. In Saccharomyces cerevisiae, the MVB pathway is composed of 17 evolutionarily conserved ESCRT (endosomal sorting complex required for transport) genes grouped by their vacuole protein sorting Class E mutant phenotypes. Only one integral membrane ...

Journal: :The Plant cell 2012
Verónica Barragán Eduardo O Leidi Zaida Andrés Lourdes Rubio Anna De Luca José A Fernández Beatriz Cubero José M Pardo

Intracellular NHX proteins are Na(+),K(+)/H(+) antiporters involved in K(+) homeostasis, endosomal pH regulation, and salt tolerance. Proteins NHX1 and NHX2 are the two major tonoplast-localized NHX isoforms. Here, we show that NHX1 and NHX2 have similar expression patterns and identical biochemical activity, and together they account for a significant amount of the Na(+),K(+)/H(+) antiport act...

Journal: :The Journal of biological chemistry 2004
Rashid Ali Christopher L Brett Sanchita Mukherjee Rajini Rao

Endosomal Na+/H+ exchangers are important for salt and osmotolerance, vacuolar pH regulation, and endosomal trafficking. We show that the C terminus of yeast Nhx1 interacts with Gyp6, a GTPase-activating protein for the Ypt/Rab family of GTPases, and that Gyp6 colocalizes with Nhx1 in the endosomal/prevacuolar compartment (PVC). The gyp6 null mutant exhibits novel phenotypes consistent with los...

2014
Xianyang Chen Hexigeduleng Bao Jie Guo Weitao Jia Fang Tai Lingling Nie Ping Jiang Juanjuan Feng Sulian Lv Yinxin Li

Despite the importance of NHX1 (Na(+)/H(+) exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of ...

Journal: :Microbiology 1999
R Nass R Rao

Osmotolerance in yeast is regulated by at least two distinct mechanisms. The acquired response occurs following long-term exposure to hypertonic medium and requires the induction of the HOG-MAP (high-osmolarity glycerol mitogen-activated protein) kinase cascade to increase levels of the osmolyte glycerol. The acute response occurs following sudden exposure to high osmotica and appears to be dep...

Journal: :Molecular biology of the cell 2000
K Bowers B P Levi F I Patel T H Stevens

We show that the vacuolar protein sorting gene VPS44 is identical to NHX1, a gene that encodes a sodium/proton exchanger. The Saccharomyces cerevisiae protein Nhx1p shows high homology to mammalian sodium/proton exchangers of the NHE family. Nhx1p is thought to transport sodium ions into the prevacuole compartment in exchange for protons. Pulse-chase experiments show that approximately 35% of t...

2001
Karen M. Wells Rajini Rao

Nhx1, the endosomal Na/H exchanger of Saccharomyces cerevisiae represents the founding member of a newly emerging subfamily of intracellular Na/H exchangers. These proteins share significantly greater sequence homology to one another than to members of the mammalian Na/H exchanger (NHE) family encoding plasma membrane Na/H exchangers. Members of both subtypes are predicted to share a common org...

Journal: :The Journal of biological chemistry 2001
K M Wells R Rao

Nhx1, the endosomal Na(+)/H(+) exchanger of Saccharomyces cerevisiae represents the founding member of a newly emerging subfamily of intracellular Na(+)/H(+) exchangers. These proteins share significantly greater sequence homology to one another than to members of the mammalian Na(+)/H(+) exchanger (NHE) family encoding plasma membrane Na(+)/H(+) exchangers. Members of both subtypes are predict...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید