We tested 103 adult Ixodes scapularis Say from 12 counties in Minnesota for the presence of Borrelia burgdorferi and the causative agent of human granulocytic ehrlichiosis (HGE), usingpolymerase chain reaction (PCR).A total of 17 ticks (16.5%)waspositive forB. burgdorferiusing nestedPCR for theßagellin gene, or bothPCR for the ospAgene andnestedPCR for theßagellin gene. A total of four ticks (3...

Trachea, lung, and conjunctive samples from 51 commercial layer farms from Bastos region, São Paulo, Brazil, were submitted to nestedPCR and virus isolation in SPF chicken embryos for ILT diagnosis. This region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. Out of the 51 tested field samples, 23 were positive for ILT by nested-PCR...

Interchromosomal recombinations between c-myc and immunoglobulin sequences can be found in preneoplastic lesions (oil granulomata) during pristane-induced plasmacytoma development in susceptible BALBkAn mice. In this study we used a more sensitiveapproach, hybridization enrichedtemplateswith nestedPCR, to detectmicrocloneswith Iga/c-myc recombinations in oil granulomata of susceptible and resis...

BACKGROUND & OBJECTIVES Appreciating how Yersinia pestis, the etiological agent of plague, spreads among black - tailed prairie dog (Cynomys ludovicianus) colonies (BTPD), is vital to wildlife conservation programs in North American grasslands. A little - studied aspect of the system is the role of Y. pestis vectors, i.e. fleas, play in the spreading of plague in natural settings. We investigat...

This study aimed to estimate the occurrence of Aedes aegypti adults at distinct climatic seasons at neighborhoods from the municipality of São Luís, Maranhão State, Brazil, as well as to verify the presence of Dengue virus (DENV) in the specimens collected. A total of 320 properties were visited in eight neighborhoods that were previously randomly chosen. Samplings were made at three periods: d...

BACKGROUND The importance of accurate diagnosis of all of major diseases cannot be underestimated and efficient laboratory testing is vital to identifying and treating life-threatening illnesses including malaria. In this study, we compared the potential of one of merozoite surface protein genes, PvMSP-3β, for detection of Plasmodium vivax in blood samples by PCR with routinely used marker, ssr...

Received: 25/Nov/2007, Accepted: 6/Feb/2008 Objective: The objective of this research was to determine the prevalence of genital C. trachomatis infection in asymptomatic women by using highly sensitive nested-polymerase chain reaction (PCR) in urine sample. Materials and Methods: One hundred-forty asymptomatic women were randomly selected from those who attended gynecology out patient departmen...

1. Generic protocol infl uenza surveillance, PAHO-CDC [cited 2011 Mar 4]. http:// www.paho.org/English/AD/DPC/CD/fl usnl-gpis.pdf 2. Panamerican Health Organization, World Health Organization. PAHO’s director newsletter. Issue 7. April 26, 2009 [cited 2011 Mar 3]. http://www.paho.org/ English/D/D_DNewsLetters_eng.asp 3. World Health Organization. WHO information for laboratory diagnosis of pand...