In the present study, zebrafish (Danio rerio) were fed casein/gelatin-based diets containing either 19% Bt (Bacillus thuringiensis)-maize or its parental non-Bt (nBt)-maize control for two generations (F0: sixty fish; F1: forty-two to seventy fish per treatment). The study focused on growth and reproductive performance, liver CuZn superoxide dismutase (SOD) enzyme activity, gene transcript leve...

Nitro blue tetrazolium polyvinyl alcohol film dosimeters, NBT-PVA were prepared and evaluated based on radiation-induced reduction of NBT2þ . NBT-PVA film dosimeters containing different concentrations of NBT dye from 1 to 5 mM were prepared in a solution of ethanol. The dosimeters were irradiated with U-ray from Co source at doses up to 50 kGy. UV/vis spectrophotometer was used to investigate ...

Neuronal bungarotoxin (NBT) is a highly selective, slowly reversible, competitive antagonist of the alpha3beta2 neuronal nicotinic receptor. Contributions to NBT sensitivity are made by both the alpha3 and beta2 subunits. We used a chimeric alpha subunit to demonstrate that the entire alpha3 contribution lies within sequence segment 84-215. Construction and analysis of a series of mutant alpha3...

The nitroblue-tetrazolium (NBT) slide test has been applied widely to assist in the diagnosis of bacterial infection. The test involves mixing a small amount of heparinized blood with a solution of NBT and noting the percentage of neutrophils that contain reduced NBT after a fixed period of incubation. Although it has been recognized that anticoagulants can influence the scores obtained, the me...

The biologic basis for the elevated histochemical reduction of nitroblue tetrazolium dye (NBT) in neutrophils from patients with acute bacterial infection or polycythemia vera was studied. A precipitin reaction followed mixing NBT with heparin. NBT was reduced after phagocytosis of this complex (H-NBT) by polymorphonuclear leukocytes (PMNs). Ingestion required divalent cations and was facilitat...

PURPOSE The aim of this study was to evaluate the ability of the NBT reduction by non- and BCG-stimulated monocytes isolated from peripheral blood persons with pulmonary tuberculosis (TB) before treatment, after two-month antituberculosis therapy and in an inactive stage of this disease. MATERIAL AND METHODS The spontaneous and induced NBT tests were done in 24 healthy individuals and 59 pati...

Nature-based therapy (NBT) has been incorporated into the practice of many medical and mental health professions. Occupational therapists, physical therapists, nurses, social workers, psychologists, and to a lesser extent, speech-language pathologists have used NBT practices as a complementary means of treating a variety of physical, emotional, and cognitive disorders. This article includes a d...

Nicotinic receptor function has been described in the retinas of a variety of vertebrate species. Neuronal bungarotoxin (NBT, also known as bungarotoxin 3.1, toxin F, or kappa-bungarotoxin) blocks nicotinic receptors in several neuronal preparations, while the neuromuscular antagonist alpha-bungarotoxin (BGT) fails to block most of these receptors. NBT (100 nM), but not BGT (10 microM), substan...

Various cellular responses to skin infections in an experimental animal model were explored. Total leukocyte counts varied after group A streptococcal infections, but a depression was commonly seen after M type 12 impetigo. Staphylococcus aureus infections resulted in moderate leukocytosis. A marked neutrophilia was universal with streptococcal or staphylococcal disease. A positive nitroblue te...

The nitroblue tetrazolium (NBT) test has been evaluated as a means of differentiating episodes of infection and rejection in 10 cadaver kidney transplant recipients. Normal NBT values were observed during all 11 episodes of acute rejection. A significant elevation of the NBT score was encountered in six of eight episodes of infection after transplantation. A positive NBT result is useful additi...