a total of 1,497 serum and blood specimens from residents of 18 different iranian communities were examined by plaque reduction neutralization test for antibodies against four (neaples, sicilian, karimabad and salehabad) phlebotomus fever virus serotypes. neutralizing antibodies against the naples and sicilian serotypes were found in every community sampled, indicating that these viruses are wi...

Viruses in the Phlebovirus genus of the family Bunyaviridae cause clinical syndromes ranging from a short, self-limiting febrile illness to fatal haemorrhagic fever. The genus currently consists of 68 antigenically distinct virus serotypes, most of which have not been genetically characterized. RT-PCR with four 'cocktail' primers was performed to amplify a region of the M segment of the genome ...

INTRODUCTION Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been ava...

little is known about sand fly fever with manifestations such as severe fever, headache and photophobia at the frontiers before the imposed war iraq against iran (1980-1988). in this article the seroepidemiological study of sand fly fever in different western provinces of iran were investigated during imposed war iraq against iran from march 1987 to march 1988. seroepidemiological studies were ...

The antigenic relationship of the 31 currently recognized phlebotomus fever group viruses was examined by plaque reduction neutralization test. Although some low-level 0cross-neutralization was observed, each of the viruses was easily differentiated by this method. Rift Valley fever virus was shown to be antigenically related to Candiru, Frijoles, Karimabad and Punta Toro viruses. Naples, Tehra...

Of the sandfly fever viruses known to be human pathogens (serotypes Toscana [TOS], Sicilian [SFS], and Naples [SFN]), only TOS has demonstrated neurotropic activity. Infections by TOS have been reported in Mediterranean countries, but the virus was previously isolated only in Italy and Portugal. We isolated 15 strains of TOS between 1988 and 1996 from the cerebrospinal fluid of patients with ac...

We conducted a serosurvey among patients of a health center in Hashimiah, a Jordanian town of 30,000 inhabitants located near a wastewater treatment plant and its effluent channel. Serum samples from 261 patients >/=5 years of age were assessed for immunoglobulin G (IgG) and IgM antibodies against West Nile, sandfly Sicilian, sandfly Naples, and Rift Valley viruses; the seroprevalence of IgG an...

et al. Evaluation of an immunoglobulin M–specifi c capture enzyme-linked immunosorbent assay for rapid diagnosis of dengue infection. To the Editor: Phleboviruses (family Bunyaviridae, genus Phlebovirus) are arthropod-borne, single-stranded, RNA viruses. Their genome consists of 3 segments—small, medium, and large—which encode the nucleoprotein and nonstructural proteins, the envelope glycoprot...

OBJECTIVE Sandfly fever phleboviruses are endemic in Mediterranean countries. We report a febrile phlebovirus case in a Greek patient who presented signs of neuroinvasive infection. METHODS In summer 2010, a 20-year-old male was admitted to hospital with fever and lethargy; he was a resident of central Macedonia, northern Greece, where a large outbreak of West Nile virus (WNV) infections occu...

Sandfly fever group viruses in the genus Phlebovirus (family Bunyaviridae) are widely distributed across the globe and are a cause of disease in military troops and indigenous peoples. We assessed the laboratory sensitivity and specificity of the Sand Fly Fever Virus Antigen Assay, a rapid dipstick assay designed to detect sandfly fever Naples virus (SFNV) and Toscana virus (TOSV) against a pan...