The use of ultrafast laser pulses in surgery has allowed for unprecedented precision with minimal collateral damage to surrounding tissues. For these reasons, ultrafast laser nanosurgery, as an injury model, has gained tremendous momentum in experimental biology ranging from in vitro manipulations of subcellular structures to in vivo studies in whole living organisms. For example, femtosecond l...

Nanosurgery using femtosecond (fs) laser pulses permits precise ablation of cellular and subcellular structures with minimal collateral damage and without compromising the cellular viability. Its nano-scale precision and minimal invasiveness allow for studying the cellular organisation from interactions of subcellular organelles to biological pathways. Fs-laser nanosurgery is versatile enough t...

The mechanics of the actin cytoskeleton have a central role in the regulation of cells and tissues, but the details of how molecular sensors recognize deformations and forces are elusive. By performing cytoskeleton laser nanosurgery in cultured epithelial cells and fibroblasts, we show that the retraction of stress fibers (SFs) is restricted to the proximity of the cut and that new adhesions fo...

Laser-mediated nanosurgery has become popular in the last decade because of the previously unexplored possibility of ablating biological material inside living cells with sub-micrometer precision. A number of publications have shown the potential applications of this technique, ranging from the dissection of sub-cellular structures to surgical ablations of whole cells or tissues in model system...

In this work we propose and build a multimodal optical workstation that extends a commercially available confocal microscope (Nikon Confocal C1-Si) to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools such as nanosurgery. The setup allows both subsystems (confocal and nonlinear) to work independently and simultaneously. The workstation enables, for instance, na...

John N Giannios1,2,3,4,5* 1President of the International Academy of Precision Medicine and Oncology 2President of the International College of Clinomics based P4 Medicine 3President of the International Association of Personalised Perioperative Medicine and Nanosurgery 4President of the Hellenic and International Society of Molecular and Genomic Medicine 5Head of the Translational Medicine Ass...