The application of nanogold in biopharmaceutical field is reviewed in this work. The properties of nanogold including nanogold surface Plasmon absorption and nanogold surface Plasmon light scattering are illustrated. The physical, chemical, biosynthesis methods of nanogold preparation are presented. Catalytic properties as well as biomedical applications are highlighted as one of the most impor...

We have systematically examined the effects of chitosan film or chitosan in combination with nanometal particles (nanogold or nanosilver particles) on the growth of Schwann cells (S16) and DRG neurons (50B 11). Our results strongly suggest chitosan and chitosan in combination with nanogold particles had no effects on the growth kinetics of Schwann cells but had slightly slowed those of DRG neur...

In the present study, invertase-mediated nanogold clusters were synthesized on onion membranes, and their application for sucrose biosensor fabrication was investigated. Transmission electron microscopy revealed free nanoparticles of various sizes (diameter ~5 to 50 nm) along with clusters of nanogold (~95 to 200 nm) on the surface of inner epidermal membranes of onions (Allium cepa L.). Most o...

Tumor-associated macrophages (TAMs) have the same immunosuppressive effects as M2 macrophages in tumor progression and are correlated with poor-patient prognosis and survival in non-small cell lung cancer (NSCLC). Therefore, TAMs are the potential targets for cancer therapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of tumor necrosis factor superfamily and sele...

Nanogold-based materials are promising candidate tools for nanobased medicine. Nevertheless, no conclusive information on their cytotoxicity is available. In the present study, we investigated the effects of gold nanoparticles (AuNPs) on human astrocytes in vitro. Nanogold treatment in a wide range of concentrations did not result in cytotoxicity. In contrast, nanogold provoked changes in the a...

This experimental study was conducted to assess of effectiveness of two nanogold concentrations against cutaneous leishmaniasis (CL) caused by Iranian strain of Leishmania major in BALB/c mice model. Cutaneous lesions were experimentally induced by inoculation with Iranian Leishmania major promastigotes (MRHO/IR/75/ER) in 61 BALB/c mice. The infected mice were enrolled to four groups: intervent...

this experimental study was conducted to assess of effectiveness of two nanogold concentrations against cutaneous leishmaniasis (cl) caused by iranian strain of leishmania major in balb/c mice model. cutaneous lesions were experimentally induced by inoculation with iranian leishmania major promastigotes (mrho/ir/75/er) in 61 balb/c mice. the infected mice were enrolled to four groups: intervent...

While electron cryotomography (ECT) provides "molecular" resolution, three-dimensional images of unique biological specimens, sample crowdedness, and/or resolution limitations can make it difficult to identify specific macromolecular components. Here we used a 1.4 nm Nanogold cluster specifically attached to the Fc fragment of IgG to monitor its interaction with the neonatal Fc receptor (FcRn),...

A new application of gold nanoparticles (AuNPs) as a colorimetric method for gene detection of α-thalassemia 1 (SEA deletion) is reported here for the first time. This technique is based on color changes from salt-induced aggregation of un-hybridized nanogold probes after hybridization with the target DNA. Specific DNA probes were synthesized, thiol modified and conjugated on the surface of AuN...

Positively charged nanogold was used as a probe to trace the internalization of plasma membrane (PM) domains carrying negatively charged residues at an ultrastructural level. The probe revealed distinct endocytic pathways within tobacco protoplasts and allowed the morphology of the organelles involved in endocytosis to be characterized in great detail. Putative early endosomes with a tubulo-ves...