The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA. To examine the most accurate and suitable methods for quantifying DNA for quality assessment, we compared three quantif...

Histopathological samples are a treasure-trove of DNA for clinical research. However, the quality of DNA can vary depending on the source or extraction method applied. Thus a standardized and cost-effective workflow for the qualification of DNA preparations is essential to guarantee interlaboratory reproducible results. The qualification process consists of the quantification of double strand D...

Biomolecular assays are continually being developed that use progressively smaller amounts of material, often precluding the use of conventional cuvette-based instruments for nucleic acid quantitation for those that can perform microvolume quantitation. The NanoDrop microvolume sample retention system (Thermo Scientific NanoDrop Products) functions by combining fiber optic technology and natura...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...

The proposed work describes the nanodrop spectrophotometric determination of cationic surfactants using citrate-modified silver nanoparticles based on the aggregation of silver nanoparticles induced by cationic surfactants due to the hydrophobic effect. The visible color change in the solution of silver nanoparticle includes a red shift with the quenching of the absorption spectra. The maximum ...

Nucleic acid extracted from environmental samples is an important analyte in instrumental analysis. With respect to the phenomenon which sodium tungstate promotes methane production anaerobic digestion, analyses of microbial community using RNA were conducted; however, we noticed that only concentration measured by absorbance measurement (NanoDrop) was remarkably high. Therefore, this study, DN...