The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two ...

A mathematical modeling of microalgae biomass is an essential step to optimize the biomass and lipid production rate and to reduce the cost of microalgal biodiesel production system. In the present study, kinetic studies were carried out to describe the growth and neutral lipid production of two marine microalgae Chlorella salina and Nannochloropsis oculata under the nitrogen-repleted and -depl...

A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20 °C versus a 15 °C to 25 °C diel temperature cycle, where light intensity also fol...

Four species of microalgae were evaluated at different temperatures for their ability to remove ammonia from intensive marine fish/shrimp culture systems. Growth rates were highest on the first of three days of culturing for marine microalgae Nannochloropsis oculata, Isochrysis aff. galbana, Chaetoceros muelleri, and Tetraselmis chui. This rate was used to compare both the growth and the total ...

Effects of an alginate oligosaccharide mixture (AOM) on Nannochloropsis oculata, a unicellular marine microalga, were investigated. The growth of N. oculata was significantly promoted by AOM in a concentration-dependent manner. The maximum effect was attained at 20 mg/ml, at which the growth rate of the alga became nearly 5 times higher than that of control without AOM. The growth-promoting eff...

Harvesting of algal biomass in biodiesel production involves high energy input and cost incurred process. In order to overcome these problems, bioflocculation process was employed and the efficiency of this process was further improved by the addition of a cationic inducer. In this work marine Bacillus subtilis was used for bioflocculation of Nannochloropsis oculata and ZnCl2 as cationic induce...

Nannochloropsis oculata is a marine-water microalgae that is considered to be a good source of omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), utilized in the production of an omega-3 oil for use as a dietary supplement. This study investigates the safety of N. oculata in male and female Sprague-Dawley rats administered a 0 or 10 mL/kg bw/rat N. oculata (10E8 viable cells/mL) sus...

background: microalgal lipid is a promising feedstock for biodiesel production. the aim of the present study was to investigate the effects of cultivation temperature on the growth and lipid accumulation properties of nannochloropsis oculata microalgae. methods: nannochloropsis oculatacan grow in a wide range of temperatures (5 ~ 35°c). late in the stationary growth phase of microalgae, biomass...

To feed Earth’s growing population, microalgae have been proposed as a source of protein and long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs). Currently, microalgae are mainly sold either as whole biomass, or the economically valuable LC n-3 PUFA fraction is extracted with organic solvents. As an alternative to solvent extraction, pH-driven protein solubilization in water followed by i...

In order to produce microalgal lipids that can be transformed to biodiesel fuel, effects of concentration of CO(2) aeration on the biomass production and lipid accumulation of Nannochloropsis oculata in a semicontinuous culture were investigated in this study. Lipid content of N. oculata cells at different growth phases was also explored. The results showed that the lipid accumulation from loga...