A sequence database was created for the Leishmania N-acetylglucosamine-1-phosphate transferase (nagt) gene from 193 independent isolates. PCR products of this single-copy gene were analyzed for restriction fragment length polymorphism based on seven nagt sequences initially available. We subsequently sequenced 77 samples and found 19 new variants (genotypes). Alignment of all 26 nagt sequences ...

background: cutaneous and visceral leishmaniases are present in fars province in the south of iran. the current study aimed to evaluate the inter- and intragenic diversities of leishmania species isolated from patients with leishmaniasis in fars province, using pcr-based analyses and dna sequencing of  the n-acetylglucosamine-1-phosphate transferase ( nagt ) gene. methods: clinical samples were...

INTRODUCTION Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death....

Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-G...

Background: Cutaneous and visceral leishmaniases are present in Fars Province in the south of Iran. The current study aimed to evaluate the inter- and intragenic diversities of Leishmania species isolated from patients with leishmaniasis in Fars Province, using PCR-based analyses and DNA sequencing of  the N-acetylglucosamine-1-phosphate transferase (nagt) gene.Methods: Clinical samples were ta...

Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but replacing part or all of this domain with leucine residues did have a dramatic ef...

Network security has become more important role today to personal users and organizations. Denial-ofService (DoS) and Distributed Denial-of-Service (DDoS) attacks are serious problem in network. The major challenges in design of an efficient algorithm in data stream are one-pass over the input, poly-log space, poly-log update time and poly-log reporting time. In this paper, we use strongly expl...

We consider an efficiently decodable non-adaptive group testing (NAGT) problem that meets theoretical bounds. The problem is to find a few specific items (at most d) satisfying certain characteristics in a colossal number of N items as quickly as possible. Those d specific items are called defective items. The idea of NAGT is to pool a group of items, which is called a test, then run a test on ...

We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giems...