Programmed proteolysis of proteins such as mitotic cyclins and Cut2/Pds1p requires a 9-residue conserved motif known as the destruction box (D-box). Strong expression of protein fragments containing destruction boxes, such as the first 70 residues of Cdc13 (N70), inhibits the growth of Schizosaccharomyces pombe at metaphase. This inhibition can be overcome either by removal of all lysine residu...

The human respiratory syncytial virus (Long strain) fusion protein contains six potential N-glycosylation sites: N27, N70, N116, N120, N126, and N500. Site-directed mutagenesis of these positions revealed that the mature fusion protein contains three N-linked oligosaccharides, attached to N27, N70, and N500. By introducing these mutations into the F gene in different combinations, four more mut...

Fusion between viral and host cell membranes is the initial step of human immunodeficiency virus infection and is mediated by the gp41 protein, which is embedded in the viral membrane. The approximately 20-residue N-terminal fusion peptide (FP) region of gp41 binds to the host cell membrane and plays a critical role in fusion catalysis. Key gp41 fusion conformations include an early pre-hairpin...

Slow Wallerian degeneration (Wld) mutant mice express a chimeric nuclear protein that protects sick or injured axons from degeneration. The C-terminal region, derived from NAD synthesizing enzyme Nmnat1, is reported to confer neuroprotection in vitro. However, an additional role for the N-terminal 70 amino acids (N70), derived from multiubiquitination factor Ube4b, has not been excluded. In wil...

There is increasing evidence that sera from HIV-1-infected individuals contain antibodies that enhance infection by HIV-1 in vitro. Previous work has demonstrated that complement receptors on T lymphoid cells and Fc receptors for IgG (Fc gamma R) on monocytic cells are required for enhanced infection by antibody-complexed HIV-1. Characterization of such infection-enhancing antibodies is essenti...

Slow Wallerian degeneration (Wld(S)) mutant mice express a chimeric nuclear protein that protects sick or injured axons from degeneration. The C-terminal region, derived from NAD(+) synthesizing enzyme Nmnat1, is reported to confer neuroprotection in vitro. However, an additional role for the N-terminal 70 amino acids (N70), derived from multiubiquitination factor Ube4b, has not been excluded. ...

Effects of enflurane on visual evoked potentials (VEP) were studied in eight healthy female patients. Control VEP were recorded from the occipital electrode (OZ-CZ), and prominent negative (N70) and positive (P100) peaks were observed following flash light stimulation. After induction of anaesthesia with thiopentone, VEP were recorded at expired concentrations of 1.2, 1.8, 2.4 and 2.7% of enflu...

Ramie (Boehmeria nivea L.) is China’s unique environmentally friendly cash crop. To protect the environment more strictly and develop sustainably, planting process will reduce input of fertilizer (nitrogen fertilizer). The effect reduced nitrogen on yield quality ramie still under systematic investigation. Taking fiber variety ‘H5’ as target, 4 N application rates 100% (N 390 kg/hm2), 85%, 70%,...

PURPOSE Transcranial direct current stimulation (tDCS) has been shown to modify the perception threshold of phosphenes elicited by transcranial magnetic stimulation (TMS). The current study was undertaken to examine whether tDCS, when applied over the occipital cortex, is also able to affect visual-evoked potentials (VEPs), which characterize occipital activation in response to visual stimulati...

The development of cardiopulmonary resuscitation by Peter Safar and others has had the unintended consequence of increasing the number of patients surviving in coma from hypoxic-ischemic encephalopathy. Neurologists often assess the prognosis for functional recovery of these patients, communicate this prognostic information to the patient’s family, and help manage the patient through the initia...