Although Mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. To address this limitation, the authors developed and optimized species-specific primer pairs that target M. eguigenitalium rpoB (RNA polymerase B subunit) gene sequences. The specificity of the PCR assay developed in this stu...

Although Mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. To address this limitation, the authors developed and optimized species-specific primer pairs that target M. eguigenitalium rpoB (RNA polymerase B subunit) gene sequences. The specificity of the PCR assay developed in this stu...

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

The ELISA and an immunoblotting technique were used to study F38-type mycoplasmas - an important cause of contagious caprine pleuropneumonia - and a number of related mycoplasma species, subspecies, types or serogroups. Two-way ELISA cross-reactivity was demonstrated between five mycoplasmas, namely strain F38, Mycoplasma mycoides subsp. mycoides (LC strain), M. equigenitalium, M. primatum and ...

one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...

a 2-year-old male pekingese dog was referred to shiraz university’s veterinary teaching hospital for anorexia and depression. the case had no history of surgery. physical examination revealed no abnormalities except mild depression and fever. small, coccoid, epicellular bacteria were detected on erythrocytes by microscopic examination of the giemsa-stained blood smears. abnormalities noted in t...

introduction: ureaplasma uriealyticum is one of the most important causes of nongonococcal and nonspecific urethritis (ngu & nsu) in men. mycoplasma hominis too has a causal role in ngu & nsu. this study aimed to investigate whether it is possible to detect mycoplasma hominis and ureaplasma uriealyticum in first voided urine samples in men suffering from ngu & nsu without complaints of urethral...