Background: Random induced mutation by gamma radiation is one of the genetic manipulation strategies to improve the antagonistic ability of biocontrol agents. Objectives: This study aimed to induce mutants with more sporulation, colonization rate leading to enhanced antagonistic ability (in vitro assay) comparing to wild type (WT) and the assessment of genetic differences (in situ evaluat...

Vol. XXXVII (August 2000), 331–350 331 *Chris Janiszewski is Jack Faricy Associate Professor of Marketing, University of Florida (e-mail: [email protected]). Stijn M.J. van Osselaer is Assistant Professor of Marketing, University of Chicago (email: [email protected]). Both authors contributed equally to this article. This work was supported by the Beatrice Foods Co. Fa...

The frequency of conversion and the disparity in the direction of conversion were studied for six frameshift mutants lying in locus b2 of Ascobolus immersus and giving more 2 wild type:6 mutant (2+6m) than 6 wild type:2 mutant (6+2m) aberrant asci (type B). The frequency of conversion decreased from left to right in the locus. The disparity steadily increased from left to right and then reached...

Interleukin-4 (IL-4) is a multifunctional cytokine that plays a critical role in apoptosis, differentiation and proliferation. The intensity of IL4 response depends upon binding to its receptor, IL-4R. The therapeutic efficiency of interleukins can be increased by generating structural mutants having greater stability. In the present work, attempts were made to increase the stability of human I...

   Ciprofloxacin is one of the most widely used antibiotics for the treatment of several infections caused by Gram-negative bacteria, like E. coli. Changes in gyrA, encoding GyrA subunit of DNA gyrase, cause the resistance to ciprofloxacin. Some ciprofloxacin resistant gyrA mutants acquired constitutive expression of marRAB operon due to the gaining mutations in marR, a repressor of this operon...

background: saccharomyces boulardii (s. boulardii) is the best known probiotic yeast. the genetic engineering of this probiotic strain requires the availability of appropriate mutants to accept various gene constructs carrying different selection markers. as the auxotrophy selection markers are under focus, we have generated a ura3 auxotroph mutant of s. boulardii for use in further genetic man...

Isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. INH-resistant mutants were shown to contain defective katG gene. Classical studies showed that INH-resistant south Indian isolates have lower virulence in guinea pigs and higher susceptibility to H2O2. It is of interest to assess the virulence in south India...

   Ciprofloxacin is one of the most widely used antibiotics for the treatment of several infections caused by Gram-negative bacteria, like E. coli. Changes in gyrA, encoding GyrA subunit of DNA gyrase, cause the resistance to ciprofloxacin. Some ciprofloxacin resistant gyrA mutants acquired constitutive expression of marRAB operon due to the gaining mutations in marR, a repressor of this operon...

In this study techniques for laboratory identification of dermatophyte fungi through protoplast hybridization were established. Firstly, auxotrophic mutants of different species of microsporum and trichophyton were induced and identified. Secondly, protoplasts from these mutants were isolated by digestion of their mycelium with Novozyme 234 using CaCI, (O.4M) as an osmotic stabilizer and g...

hepatitis b virus (hbv) is a crucial public health problem with approximately 350 million affected people and a death rate of 0.5 to 1.2 million per year worldwide. it proceeds to end-stage liver diseases including cirrhosis with hepatic decompensation and hepatocellular carcinoma (hcc). pakistan lies in the endemic region with 3% hbv carrier rate in the country. the differences in disease outc...