introduction: one of the major contributing factors, which may cause failure of endodontic treatment, is the presence of residual microorganisms in the root canal system. for years, most dentists have been using calcium hydroxide (ch) as the intracanal medicament between treatment sessions to eliminate remnant microorganisms. reducing the size of ch particles into nanoparticles enhances the pen...

Peritoneal macrophages from mice infected with Bacille Calmette-Guérin (BCG) and from normal mice were examined for their effects in vitro on thymidine uptake by 10 murine lymphomas, a murine fibroblast line, and a guinea pig hepatoma. Only the murine fibroblast line showed growth inhibition in the presence of BCG macrophages. For the majority of tumors, normal macrophages were profoundly stimu...

Hybrids constructed with the murine fibroblasts IT22 and either human lymphoid cells or human melanoma cells retain the human chromosomes 1, 14, and 21 and lose the human chromosome 2. The human lymphocyte-mouse fibroblast hybrids preferentially retain the human chromosome 11, while the human melanoma-murine fibroblast hybrids preferentially retain the human chromosome 8 and lose the human chro...

A convenient synthesis of indolin-2-ones substituted in the 3 position by an aminomethylene group bearing different amino acid moieties is described. Their antiproliferative activities were evaluated toward a panel of human solid tumor cell lines (PC 3, DLD-1, MCF-7, M4 Beu, A549, PA 1) and healthy cell lines (a murine fibroblast L929 and a human fibroblast primary culture).

Cardiac fibroblasts have been long recognized as active participants in heart disease; however, their exact physiological and pathological roles remain elusive, mainly due to the lack of specific markers. In this issue of the JCI, Moore-Morris and colleagues used a fibroblast-specific collagen1a1-GFP reporter to demonstrate that fibroblast accumulation after aortic banding in murine hearts aris...

A previous report has shown a deficiency of a cholinergic differentiating factor in spent culture media in which Alzheimer's disease (AD) patient fibroblasts were grown (Kessler, 1987). We used a similar approach to investigate whether AD fibroblast-conditioned medium demonstrated central nervous system (CNS) neuronal growth sustaining properties. For these investigations we used cultured fetal...

Rationale: Lymphangioleiomyomatosis (LAM) is a multisystem disease that causes lung cysts and respiratory failure. Loss of TSC (tuberous sclerosis complex) gene function results in clone “LAM cells” with dysregulated mTOR (mechanistic target rapamycin) activity. LAM cells fibroblasts form nodules also contain mast cells, although their significance unknown. Objectives: To understand the mechani...