two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of sulfamethoxazole (smx) and phthalazine (phz) by high performance liquid chromatography (hplc). multivariate calibration techniques such as classical least squares (cls) and inverse least squares (ils) were introduced into hplc to determine the quantification by using uv detecto...

a new method was developed for the spectral resolution by further determination of three- and  four-component  mixtures  of  drugs in urine samples through  the complementary application of multivariate curve resolution-alternating least squares with correlation  constraint. in the current study, a simple method was proposed to construct a calibration set for the mixture of drugs in the presenc...

A new method for multivariate calibration [1 – 3] eliminates many of the problems associated with traditional methods, making the process easier and more transparent to the user. It uses physical methods to estimate the spectral signal and statistical methods to estimate noise. It is also less expensive to apply than partial least squares (PLS) or principal component regression (PCR) calibratio...

the multivariate calibration method, partial least square regression (pls) was applied for thesimultaneous spectrophotometric determination of lidocaine (lid) and hydrocortisone acetate(hca) in their mixtures. the parameters of chemometric technique were optimized and theproposed method was validated with synthetic samples and applied to analyze these drugs inpharmaceutical products with good a...

We present a hybrid multivariate calibration method, constrained regularization (CR), and demonstrate its utility via numerical simulations and experimental Raman spectra. In this new method, multivariate calibration is treated as an inverse problem in which an optimal balance between model complexity and noise rejection is achieved with the inclusion of prior information in the form of a spect...

1. Methods to be considered for multivariate calibration Many methods for multivariate calibration have been proposed. It turns out that many of the methods perform similarly. To avoid confusion due to use of many different methods, it is suggested that only the following should be considered: Multiple linear regression (MLR) Principal component regression (PCR) Partial least squares (PLS) Neur...

      A new method was developed for the spectral resolution by further determination of three- and  four-component  mixtures  of  drugs in urine samples through  the complementary application of multivariate curve resolution-alternating least squares with correlation  constraint. In the current study, a simple method was proposed to construct a calibration set for the mixture of drugs in the p...

attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. in the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...