the growing demand for animal products has made importance of the utilization of biotechnological techniques in improvement of forage crops such as sainfoin. in this research, the effects of different levels of auxin (naa and iba) and cytokinin (bap, zeatin and tdz) on in vitro growth response of shoot apex explants of shahrkord and khansar cultivars of sainfoin were investigated. for this, sho...

in vitro propagation of rose (rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including indian subcontinent. the development of new cultivars for commerce is faced with new challenges. in vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

To develop an ef®cient procedure for plant regeneration from leaf-derived callus of Lavandula vera DC, the production of multiple shoots and the formation of roots from these shoots were studied. When calli were cultured in a medium with 4.0 10ÿ7 M N-(2-chloro-4-pyridyl)-N0phenylurea (CPPU), urea-type cytokinin, multiple shoots were obtained from the greenish surface of the callus ef®ciently at...

Multiple shoots were induced from axillary and apical buds of Crossandra infundibuliformis (L.) on MS medium containing BAP and kinetin individually. The maximum number of multiple shoots were obtained from MS medium containing BAP 1 mgÿ1. The subcultures were done on fresh medium with the same composition and the number of multiple shoots increased during subculture of both types of explant. A...

In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at l...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

In vitro studies were carried out with Ochradenus arabicus for rapid micropropagation from embryo explants. Direct regeneration of shoot buds of O. arabicus was observed in MS basal medium supplemented with varying levels of either benzyladenine (BA) or kinetin (Kn). Embryos produced multiple shoots on Murashige and Skoog’s medium (MS) containing 1.0 μM BA after 25 days of inoculation. Number o...

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

An efficient and reproducible in vitro protocol for largescale multiplication of Liquorice (Glycyrrhiza glabra L.)has been described. Multiple shoots formation was significantly influenced by growth regulators, photoperiod,explant position, season of explant collection and culture passage. Nodal explants were collected atmonthly intervals to initiate in vitro cultures. The hig...