Several methods have been described for the determination of urinary acid mucopolysaccharides (1-4). It has been found that in normal individuals the daily urinary excretion varies with age and sex (4, 5). A few attempts have been made to correlate urinary excretion of acid mucopolysaccharides with different disease states. Craddock and Kerby (6) have reported that patients with impairment of r...

Several methods have been described for the determination of urinary acid mucopolysaccharides (1-4). It has been found that in normal individuals the daily urinary excretion varies with age and sex (4, 5). A few attempts have been made to correlate urinary excretion of acid mucopolysaccharides with different disease states. Craddock and Kerby (6) have reported that patients with impairment of r...

On Ecteola columns in 0.05M HCl (pH 1.3) the sulfate groups of mucopolysaccharides are. dissociated while the carboxyl groups are not. In 0.05M acetate buffer (pH 4.8), the carboxyl groups are dissociated and those mucopolysaccharides which do not bind, to Ecteola at the loioer pH can be resolved. These properties have been utilized, to resolve mixtures of beef retina and small beef cerebral bl...

Proteoglycans of 300 000 mol.wt. were isolated from dispersed rat basophil tumour cells after labelling of the sulphated mucopolysaccharides with 35S in vitro:90% of the 35S-labelled mucopolysaccharides were extracted at high salt concentration. Alkali degradation of the 35S-labelled proteoglycans yielded glycosaminoglycan chains of 40 000 mol.wt. The composition of the salt-extracted 35S-label...

Increased attention has been focused on the potential significance of the variations in the qualitative and quantitative composition of connective tissue ground substance. Changes in tissue content of acid mucopolysaccharides are difficult to define since existing quantitative methods are generally recognized as inadequate. Moreover, these methods are laborious and require large amounts of star...

Heparin and other acid mucopolysaccharides were extracted from various tissues by a modification of the method previously described (Bassiouni, 1953). The dye mucopolysaccharides complex obtained was resolved into its components with formamide: the acid mucopolysaccharides were precipitated with methyl alcohol in the presence of electrolyte (sodium chloride). A solution of the purified polysacc...

The effect of dibutyryl-cyclic adenosine 3’,5’-monophosphate and theophylline on the incorporation of [%]sulfate into acid mucopolysaccharides by 3T3 and SV3T3 fibroblasts has been studied. SV40 transformation of 3T3 fibroblasts produced a marked decrease in the rate of synthesis of acid mucopolysaccharides, in agreement with the data of others. When the transformed cells were grown in medium s...

It has been known that the mucihematein stain of Mayer (1896) is a traditional staining method for mucin. Thompson (1966) has noted that Mayer introduced the mucihematein stain for the demonstration of certain types of acid mucopolysaccharides in tissue sections. Although this technic is relatively specific it is empirical. According to Mallory (1938), Mayer's mucihematein stain is particularly...

A clonal strain of rat glial tumor cells was shown to produce the acid mucopolysaccharides, hyaluronic acid, chondroitin 4-SO(4), and heparan sulfate in tissue culture. The mucopolysaccharides were isolated both from the growth medium and the cells.

1. The uptake of dilute neutral hypochlorite by epithelial mucopolysaccharides has been compared with that of proteins, polysaccharides, amino acids and sugars. The uptake has been shown to be related to the protein content of the mucopolysaccharides rather than their polysaccharide content. 2. The destruction of the components of epithelial mucopolysaccharides, certain sugars and polysaccharid...