while the oecd test guidelines and mostly animal assays have been used to study the toxic effects of chemicals for many years, very little is known about the potential toxicity of vast majority of inhaled chemicals. considering large number of chemicals and complex mixtures present in indoor and outdoor air, heavy reliance on animal test methods appear to be not adequate. continuing scientific ...

The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to full automation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier. Estimation requires only 5 mm. of incubation, and assays may be run at th...

A colorimetric method for measuring the viability of Leishmania promastigotes is described that is based on the reduction of the tetrazolium salt, XTT, to a water-soluble formazan. Values obtained by the XTT method correlated well with parasite number (r=0.965) and with methods that rely upon the reduction of MTT or MTS (r=0.96 and 0.97, respectively). The IC(50) values obtained by XTT method w...

The effective microorganism (EM-X) fermentation extract is derived from rice bran and seaweed extract. It has been shown to possess anti-oxidation activity both in vitro and in vivo. To our knowledge, the possible in vitro anti-cancer potential of EM-X has not been demonstrated. Here we showed that the double concentrate of EM-X (EM-X2) at concentrations of 20-30% by volume, had growth inhibito...

Background It is of great clinical significance to further explore new strategies and potential combined therapeutic targets for gastric cancer. This study aimed investigate the synthetic lethal effect RBBP8 molecular intervention with a poly ADP ribose polymerase (PARP) inhibitor in non-BRCA mutant cancer clarify mechanism by which regulates homologous recombination repair. Methods The role DN...

The dynamic instability of cortical microtubules (MTs) (i.e., their ability to rapidly alternate between phases of growth and shrinkage) plays an essential role in plant growth and development. In addition, recent studies have revealed a pivotal role for dynamic instability in the response to salt stress conditions. The salt stress response includes a rapid depolymerization of MTs followed by t...

BACKGROUND Without appropriate cellular models the etiology of idiopathic Parkinson's disease remains unknown. We recently reported a novel patient-derived cellular model generated from biopsies of the olfactory mucosa (termed olfactory neurosphere-derived (hONS) cells) which express functional and genetic differences in a disease-specific manner. Transcriptomic analysis of Patient and Control ...

  Background : Many studies have focused on oxidative stress induced damage and hence, the protective effects conferred by antioxidants. An example is neurodegenerative diseases which is thought to occur due to neuronal loss associated with oxidative stress. However, some antioxidants such as vitamin E have been shown to also exert pro-oxidative effects at high concentration.   Objective : In t...