cancer is a major global problem and is the second leading cause of mortality in the developed countries.resistance to current chemotherapeutics and high incidence of adverse effects are the two principal reasons for developing new anticancer agents. phenylacetamide derivatives can act as potential anticancer agents. synthesis and screening of 2-(4-fluorophenyl)-n-phenylacetamide derivatives in...

a multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (sf) of irradiated cells. the estimation of sf based on the cell‑growth curve information is the major advantage of this assay. in this study, the utility of multiple‑mts assay for the sf estimation of irradiated ht‑29 colon cancer cells, which were plated before irradiation, w...

The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Thre...

The central spindle forms between segregating chromosomes during anaphase and is required for cytokinesis. Although anaphase-specific bundling and stabilization of interpolar microtubules (MTs) contribute to formation of the central spindle, it remains largely unknown how these MTs are prepared. Using live imaging of MT plus ends and an MT depolymerization and regrowth assay, we show that de no...

Microtubule-associated protein 4 (MAP4) promotes MT assembly in vitro and is localized along MTs in vivo. These results and the fact that MAP4 is the major MAP in nonneuronal cells suggest that MAP4's normal functions may include the stabilization of MTs in situ. To understand MAP4 function in vivo, we produced a blocking antibody (Ab) to prevent MAP4 binding to MTs. The COOH-terminal MT bindin...

Despite extensive studies, the molecular mechanisms of Tau binding to microtubules (MTs) and its consequences on MT stability still remain unclear. It is especially true in cells where the spatiotemporal distribution of Tau-MT interactions is unknown. Using Förster resonance energy transfer (FRET), we showed that the Tau-MT interaction was distributed along MTs in periodic hotspots of high and ...

We evaluated the effect of a basic fibroblast growth factor (bFGF) and saporin conjugate (bFGF-SAP) on proliferation, migration and tubule formation in bovine choriocapillary endothelial cells (BCECs). Cell proliferation and MTS assays were done with 0.01, 0.1, 1, 10, and 100 nM bFGF-SAP, and an equimolar concentration of bFGF and saporin. TUNEL assay was performed to confirm apoptosis. Cells w...

A recombinant metallothionein CgMT1, from the Pacific oyster Crassostrea gigas, was synthesized and used as antigen in the development of antibodies and a specific enzyme-linked immunosorbent assay (ELISA). The ELISA showed that rabbit anti-CgMT1 IgG reacted with purified CgMT1 and MTs from other marine bivalves, indicating its suitability as a reagent to quantify MTs and for monitoring of meta...

Tobacco smoking is associated with cardiovascular pathology. However, the molecular mechanisms of tobacco smoke exposure that lead to initiation or exacerbation of cardiovascular disease are unclear. In this study, the effects of mainstream tobacco smoke (MTS) on global transcription in the heart were investigated. Male C57B1/CBA mice were exposed to MTS from 2 cigarettes daily, 5 days/wk for 6...

neuroinflamation occurring after traumatic brain injury (tbi) is a complex phenomenon comprising distinct cellular and molecular events involving the injured cerebral tissue. mechanical injury to adult rat brain elucidated the accumulation in the affected area of trophic activity. in the tissue adjacent to the injury, trophic titers began to rise after the lesion. this study aimed to determine ...