conclusions mtb32c and hbha genes were successfully isolated from h37rv genome and cloned into an eukaryotic expression vector. this vector can be considered as a vaccine to evaluate immune responses in animal models. results in the current study, recombinant pcdna3.1 (+) vector containing mtb32c and hbha genes was successfully constructed. the desired size of dna fragment was observed using si...

Recombinant immunodominant mycobacterial antigens are needed for the development of new vaccines and immunodiagnostic tools for use against tuberculosis. Ubiquitous exposure to mycobacteria in tropical countries could influence vaccine-induced immunity and the specificity of tuberculosis immunodiagnosis. For this study conducted in The Gambia, cellular immune responses to recombinant mycobacter...

Key Ags of Mycobacterium tuberculosis initially identified in the context of host responses in healthy purified protein derivative-positive donors and infected C57BL/6 mice were prioritized for the development of a subunit vaccine against tuberculosis. Our lead construct, Mtb72F, codes for a 72-kDa polyprotein genetically linked in tandem in the linear order Mtb32(C)-Mtb39-Mtb32(N). Immunizatio...

Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading fatal infectious diseases. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, three candidate antigens, ESAT-6 (6 kDa early secretory antigenic target) and Mtb72F (a fusion polyprotein from two TB antigens, Mtb32 and Mtb39) fused with ch...

Recent advances in molecular and genomic techniques have facilitated research on several aspects of mycobacteriology, such as diagnosis and the identification of new vaccines and therapeutic targets for various diseases, including tuberculosis. The aim of this review was to analyze the implications of advances in molecular and genomic techniques on the development of new vaccines for tuberculos...

background: hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of echinococcus granulosus. diagnosis of hydatidosis is still an unresolved problem. serological tests using crude antigens for diagnosis of e. granulosus are sensi-tive, however their specificity are not satisfactory. therefore, who recommended spe-cific serological methods using specific antigens,...

objective(s): the aim of this study was to estimate effects of hyperbaric (hb) treatment by determination of cd15s and cd11b leukocyte proinflammatory markers expression.  in addition, this study describes changes in cd77 and cd34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. materials and methods:expression of cd11b and cd...

capture elisa system was developed for diagnosis of visceral leishmaniasis (vl) using a monoclonal antibody raised against an antigen previously detected in the urine of vl patients. urine samples from confirmed vl cases from yemen, nepal, spain, sudan and brazil were tested in the capture elisa in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...