This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DC...

this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

Induction of protein synthesis by the external delivery of in-vitro transcription-messenger RNA (IVT-mRNA) has been a useful approach in the realm of cell biology, disease treatment, ‎reprogramming of cells, and vaccine design. Therefore, the development of new formulations for ‎protection of mRNA against nucleases is required to maintain its activity in-vivo. It was the aim o...

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its in CHO-K1 cells and the inhibition effect pig on PRRSV proliferation verified. The plasmid pEGFP-PoIFN-λ1 from gene fragment transfected into cells. Expression detected by fluorescence microscopy Western blotting. influence assessed. results study showed that recombinant correctly. After transfection, green f...

In vitro-transcribed (IVT) mRNA has come into focus in recent years as a potential therapeutic approach for the treatment of genetic diseases. The nebulized formulations IVT-mRNA-encoding alpha-1-antitrypsin (A1AT-mRNA) would be highly acceptable and tolerable remedy protein replacement therapy deficiency future. Here we show that lipoplexes containing A1AT-mRNA prepared optimum conditions coul...

An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there need an optimized process individual model systems separately. Mouse pancreatic αTC1-6 which act as attractive system cell reprogramming diabetes treatment, were transiently transfected with two different methods: chemical method polyethyleneimine (PEI) nucleofection physical met...

Objective: To investigate the expression of miR-642 in gastric cancer and its mechanism proliferation migration. Methods: qRT-PCR was used to detect cancer. Dual luciferase gene reporting system verify direct regulatory effect on KRT19 3' UTR. The mRNA protein levels after transfection were detected by Western blot. IncucyteS long term dynamic cell observation. Kaplan-Meier method analyze relat...

Background: Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ab...