N-methylpurine DNA glycosylase (MPG), a DNA repair enzyme, functions in the DNA base excision repair (BER) pathway. Aberrant over-expression of MPG in various cancers suggests an important role of MPG in carcinogenesis. Identification of MPG-interacting proteins will help to dissect the molecular link between MPG and cancer development. In the present study, using immunoprecipitation coupled wi...

BACKGROUND MPG is a cell-permeable peptide with proven efficiency to deliver macromolecular cargoes into cells. In this work, we examined the efficacy of MPG as an N-terminal tag in a fusion protein to deliver a protein cargo and its mechanism of transduction. RESULTS We examined transduction of MPG-EGFP fusion protein by live imaging, flow cytometry, along with combination of cell biological...

BACKGROUND Whether myocardial perfusion grade (MPG) following late recanalization of infarct-related arteries (IRAs) predicts left ventricular (LV) function recovery beyond the acute phase of myocardial infarction (MI) is unknown. METHODS AND RESULTS The Total Occlusion Study of Canada-2 enrolled stable patients with a persistently occluded IRA beyond 24 hours and up to 28 days post-MI. We st...

AIM To examine the expression of N-methylpurine-DNA glycosylase (MPG) gene and protein in glioma samples with different WHO grades and its association with patients' survival. METHODS Immunohistochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues. RESULT...

Body DWI has been proposed recently as a sensitive tumour detection method. One of the major issues with abdominal DWI is signal loss in soft tissues. Recent study (1) showed that this is mainly due to cardiac pulsation. Voxel deformation due to cardiac motion combined with strong diffusion gradient pulse induces intra-voxel spin phase dispersion that causes signal loss in both left and right l...

When diffusion tensor was analyzed, increasing motion probing gradient (MPG) directions and increasing signal-to-noise ratio (SNR) as result might influence diffusion tensor calculation. This study determined that mean diffusivity (MD) and fractional anisotropy (FA) are calculated by changing MPG-directions or SNR in the volunteer. There were no statistically significant differences in MD, whic...

BACKGROUND Valve effective orifice area EOA and transvalvular mean pressure gradient (MPG) are the most frequently used parameters to assess aortic stenosis (AS) severity. However, MPG measured by cardiovascular magnetic resonance (CMR) may differ from the one measured by transthoracic Doppler-echocardiography (TTE). The objectives of this study were: 1) to identify the factors responsible for ...

OBJECTIVE The A1C-Derived Average Glucose (ADAG) study demonstrated a linear relationship between HbA(1c) and mean plasma glucose (MPG). As glucose variability (GV) may contribute to glycation, we examined the association of several glucose variability indices and the MPG-HbA(1c) relationship. RESEARCH DESIGN AND METHODS Analyses included 268 patients with type 1 diabetes and 159 with type 2 ...

OBJECTIVE This study examined the role of SNP rs2858056 of the MPG gene on the incidence and severity of rheumatoid arthritis (RA). METHODS This cohort study enrolled 365 RA patients and 375 age- and gender-matched healthy controls, all of whom had Han Chinese ethnicity and were from Taiwan. Gene polymorphism of the SNP rs2858056 of MPG was determined from genomic DNA. Allelic frequencies and...

INTRODUCTION AND OBJECTIVES The relationship between microcirculatory myocardial perfusion grade (MPG), myocardial salvage and long-term mortality after acute ST-segment elevation myocardial infarction (STEMI) and full restoration of epicardial blood flow by primary percutaneous coronary intervention (PCI) remains poorly understood. METHODS This study included 1213 patients with STEMI and Thr...