نتایج جستجو برای: mouse oocytes
تعداد نتایج: 298591 فیلتر نتایج به سال:
background: the present study has been carried out to investigate the effects of preovulatory stage gamma-irradiation of female mice in the absence or presence of vitamin e on numerical chromosome abnormalities in 8-cell embryos after mating with non-irradiated males. materials and methods: the 8-11 weeks adult female nmri mice were whole body irradiated at preovulatory stage (post pmsg injecti...
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objective: phthalates, which are commonly used to render plastics into soft and flexible materials, have also been determined as developmental and reproductive toxicants in human and animals. the purpose of this study was to evaluate the effect of mono-(2- ethylhexyl) phthalate (mehp) and di-(2-ethylhexyl) phthalate (dehp) oral administrations on maturation of mouse oocytes, apoptosis and gene ...
Background: Vitrification of oocyte is one of the most important topics in the field of assisted reproductive techniques (ART). Considering the importance of oocyte vitrification in clinics, in the present study the effect of vitrification on mouse oocytes survivale rate and apoptosis by cryotop were investigated. Materials and Methods: 200 GV oocytes and 200 MII oocytes were obtained respectiv...
Background and Purpose: In vitro maturation of oocyte is a promising technology in treatment of infertility, however, its clinical application is still limited due to poor success rate. This study was devised to evaluate the effect of mouse embryo tail bud co-culture on maturation of immature mouse oocyte. Materials and Methods: Immature oocytes were recovered from NMRI female mice 48hr after ...
Purpose: This Study evaluated the resumption of meiosis, in vitro maturtion and department of immature mouse oocytes with b- mercaptothanol. Materials and methods: Immature oocytes were recovered from NMRI mouse strain (4-6 weeks) in different 4 experiments groups: the control group, the oocytes were matured in MEM-a+ medium containing + FCS 55%; group 1: the oocytes were matured in MEM- a med...
Background An evaluation of the developmental competence of vitrified mouse germinal vesicle (GV) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. MaterialsAndMethods Mouse GV oocytes were considered in cumulus-denuded oocytes (CDOs) and cumulus-oocyte complexes (COCs) g...
Purpose: To study the effect of ethanol on resumption of meiosis, maturation and development of immature mouse oocytes in vitro. Materials and Methods: Immature oocytes were dissected from ovary of NMRI strain mouse (6-8 weeks old) and divided into control and experimental groups. The absolute ethanol was imposed to oocytes at doses of 0 (Control), 0.05, 0.1, 0.2 and 0.4% (v/v) in maturation c...
Purpose: The purpose of this study was to develop an appropriate medium for in vitro maturation (IVM) of immature mouse oocytes. Materials and Methods: Germinal vesicle of female NMRI mouse oocytes (6-8 weeks old) were collected from ovaries and cultured in maturation medium MEM-a, supplemented with: 100 mlIU/ml rFSH+7.5 IU/ml hCG+5% FCS (Control group) and 2mM all- trans retinoic acid (t-RA) ...
background: artificial stimulation of mouse oocyte, in the absence of sperm contribution, can induce its parthenogenic activation of oocyte. ultrasound is one of the newest methods for artificial activation of mammal oocytes, and its successful utilization in pig oocyte activation has been recently reported. our objective was to assess the effect of ultrasound on mouse oocyte activation. materi...
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