currently, different highly sensitive nucleic acid detection-based protocols used in medical diagnostic laboratories need specific working conditions, and if no optimization is performed, misleading results can be obtained. limitations of each protocol is different in obtaining an appropriate result. awareness of these limitations plays an important role in providing a reliable result for physi...

molecular diagnostic methods have played and continuing to have a critical role in clinical laboratories in recent years. therefore, standardization is an evolutionary process that needs to be upgrade with increasing scientific knowledge, improvement of the instruments and techniques. the aim of this study was to design a quality assurance program in order to have similar conditions for all med...

DNA size markers (ladder) are essential tools in molecular biology, genetics and biotechnology. In this study, a simple and cost-effective method for laboratory production of DNA ladders is introduced. For this purpose, different sizes of 100 to 2000 bp DNA segments were designed using PCR technique. For producing 14 different gene fragments as DNA molecular weight markers, recombinant plasmid ...

Technologies in available biomedical repositories do not yet provide adequate mechanisms to support the understanding and analysis of the stored content. In this project we investigate this problem under different perspectives. Our contribution is the design of computational solutions for the analysis of biomedical documents and images. These integrate sophisticated technologies and innovative ...

What is it famous for? Being a successful, truly European research centre — still a very rare species. Early plans for EMBL were much influenced by the most famous of the species, the Conseil Européen pour la Recherche Nucleaire (CERN), and by Leo Szilard, a nuclear physicist turned molecular biologist. Where is it? About 80% of EMBL group leaders are at the main centre, on top of a beautiful b...

Methodology For the selection of the markers, a reference population of 30 unrelated parents for each species of interest (Eucalyptus globulus and Pinus radiata) was used. In these populations, we tested 25 SSR for pinus and 38 SSR for eucalyptus and the best 12 markers were selected for each species. With the selected markers, a pattern or fingerprint of each operational clone was obtained, ou...