abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

aucuparia is an important slow growth forest tree with medicinal, industrial and ornamental uses which is applied for reforestation in high altitude of mountain lands. this species has been endangered at northern forests of iran; therefore micropropagation of adult trees by bud culture may help to reforestation. the best sterilizing treatment was the buds washing with hgcl2% 0.1 solution for 7 ...

Aucuparia is an important slow growth forest tree with medicinal, industrial and ornamental uses which is applied for reforestation in high altitude of mountain lands. This species has been endangered at northern forests of Iran; therefore micropropagation of adult trees by bud culture may help to reforestation. The best sterilizing treatment was the buds washing with HgCl2% 0.1 solution for 7 ...

SummaryAim. To optimize the processes of microclonal propagation Juglans regia in vitro by selecting composition and consistency nutrient medium. Methods. The methods culture establishment initial explants were used. Parts stem buds young sprouts, germinated mini-greenhouse method from seeds regia, used as explants. Murashige&Skoog (MS) Driver&Kuniyuki (DKW) media to establish vitro. Ag...

Micropropagation of hemp ( Cannabis sativa ) is constrained by problems with hyperhydricity and culture decline microshoots. These can be reduced increasing agar nutrients in the media during micropropagation stages 1 2, respectfully. Performance microshoots ‘Abacus’ ‘Wife’ cultured Driver Kuniyuki Walnut medium (DKW) for 15 weeks (6 stage + 9 2), subculturing every 3 both or Murashige Skoog vi...

this research was conducted to study the factors affecting establishment, growth and shoot proliferation of persian walnut, genotype z60. explant establishment was studied through a factorial experiment based on a completely randomized design with two factors including culture media (dkw and wpm) and ba concentration (0 (control), 0.5 and 1.0 µm). single-node explants were prepared from the you...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

Pruacan is a rare medicinal plant endemic to the Dieng Plateau of Central Java, Indonesia which has properties as an aphrodisiac. Increasing genetic variability ofthis in order support breeding programs and increase productivity active ingredients this important. This study aimed evaluate effect chemical mutagen EMS on growth development vitro culture pruacan lethal dose determination. Embryoge...

this study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of gisela 6 rootstock at khorasan razavi agriculture and natural resource research center during 2011-2012. in this experiment, the proliferation and rooting were performed in six kinds of culture medium (...