objective(s): increased levels of nitric oxide (no) in the testicular veins of people suffering from varicocele have already been reported. however, the role of no-synthase (nos) isozymes and their inhibitors have not been extensively studied. we aimed to evaluate the inhibitory effects of aminoguanidine (ag), on sperm motility, vitality, and mitochondrial membrane potential (mmp) in varicoceli...

dacarbazine is an antitumor prodrug which is used for the treatment of malignant metastatic melanoma and hodgkin’s disease. it requires initial activation in liver through an n-demethylationreaction. the active metabolite prevents the progress of disease via alkylation of guanine bases in dna strands. in order to investigate the importance of imidazole ring and its dynamictautomerization in ant...

mitochondrial functions have a major impact on t-cell functionality. in this study we characterized whether mitochondrial function in the neonatal t-cells differs from that in the adult t-cells during short t-cell activation. methods: we used fow cytometry methods to test mitochondrial mass and to monitor mitochondrial ca2+ levels, mitochondrial potential and superoxide generation in parallel w...

overdose of acetaminophen causes severe hepatic necrosis in humans and experimental animals. studies on its hepatotoxicity remain a very active area since some of current data are still uncertain. in this study, freshly isolated rat hepatocytes were used to determine the effects of garlic extract and its component, allicin on the acetaminophen-induced cell cytotoxicity and to compare with the e...

background: spermatozoa cryopreservation is used for the management of infertility and some other medical conditions. the routinely applied cryopreservation technique depends on permeating cryoprotectants, whose toxic effects have raised the attention towards permeating cryoprotectants-free vitrification technique. objective: to compare between the application of slow cryopreservation and vitri...

Cigarette smoking is one of the main risk factors for premature human death associated to a variety of respiratory and vascular diseases, and cancer due to containing Hundreds of toxicants. Rat mitochondria were obtained by differential ultracentrifugation and incubated with different concentrations (1, 10 and 100%) of standardized cigarette smoke extract (CSE). Our results showed that cigarett...

chloroacetaldehyde (caa) is a chlorination by-product in finished drinking water and a toxic metabolite of a wide variety of industrial chemicals (e.g. vinyl chloride) and chemotherapeutic agents (e.g. cyclophosphamide and ifosfamide). in this research, the cytotoxic mechanisms of caa in freshly isolated rat hepatocytes were investigated.caa cytotoxicity was associated with reactive oxygen spec...

Chloroacetaldehyde (CAA) is a chlorination by-product in finished drinking water and a toxic metabolite of a wide variety of industrial chemicals (e.g. vinyl chloride) and chemotherapeutic agents (e.g. cyclophosphamide and ifosfamide). In this research, the cytotoxic mechanisms of CAA in freshly isolated rat hepatocytes were investigated.CAA cytotoxicity was associated with reactive oxygen spec...

Chloroacetaldehyde (CAA) is a chlorination by-product in finished drinking water and a toxic metabolite of a wide variety of industrial chemicals (e.g. vinyl chloride) and chemotherapeutic agents (e.g. cyclophosphamide and ifosfamide). In this research, the cytotoxic mechanisms of CAA in freshly isolated rat hepatocytes were investigated.CAA cytotoxicity was associated with reactive oxygen spec...

cigarette smoking is one of the main risk factors for premature human death associated to a variety of respiratory and vascular diseases, and cancer due to containing hundreds of toxicants. rat mitochondria were obtained by differential ultracentrifugation and incubated with different concentrations (1, 10 and 100%) of standardized cigarette smoke extract (cse). our results showed that cigarett...