background: methionine has been suggested as the firstlimitingamino acid for milk production in ruminants. it is importanthow to increase milk protein yield and milk fat in dairy ruminants.objectives: this study was set to investigate the effect of rumenprotected methionine (rpmet) on milk composition of lactatingcashmere rayeni goats. methods: 40 healthy singletoncashmere rayeni goats about 3 ...

A two-dimensional thin-layer chromatographic system that separates all of the known polar lipids of milk and mammary tissue is described. The compositions of phospholipids of milk and of the mammary tissue from which the milk was derived are presented.

The technology is now available for commercial cloning of farm animals for food production, but is the food safe for consumers? Here, we provide data on >100 parameters that compare the composition of meat and milk from beef and dairy cattle derived from cloning to those of genetic- and breed-matched control animals from conventional reproduction. The cloned animals and the comparators were man...

The isolation of whey proteins from human and bovine milks followed by profiling of their entire N-glycan repertoire is described. Whey proteins resulting from centrifugation and ethanol precipitation of milk were treated with PNGase F to release protein-bound N-glycans. Once released, N-glycans were analyzed via nanoflow liquid chromatography coupled with quadrupole time-of-flight mass spectro...

Human milk oligosaccharides are key components of human milk and appear in various compositions and concentrations in all human milks. In regulatory sense human milk oligosaccharides are classified as novel foods or novel food ingredients requiring safety assessment. In addition, if any health messages are intended to be used also health claim regulations apply. This chapter reviews the regulat...

BACKGROUND Airag, alcoholic sour-tasting beverage, has been traditionally prepared by Mongolian nomads who naturally ferment fresh mares' milk. Biochemical and microbiological compositions of airag samples collected in Ulaanbaatar, Mongolia and physiological characteristics of isolated lactic acid bacteria were investigated. METHODS Protein composition and biochemical composition were determi...

Secretory immunoglobulin A (sIgA) is a major glycoprotein in milk and plays a key role in mediating immune protection of the gut mucosa. Although it is a highly glycosylated protein, its site-specific glycosylation and associated glycan micro-heterogeneity have still not been fully elucidated. In this study, the site-specific glycosylation of sIgA isolated from human colostrum (n = 3) was analy...

There is a steady increasing demand for insulin worldwide. Current insulin manufacturing capacities can barely meet this increasing demand. The purpose of this study was to test the feasibility of producing human proinsulin in the milk of transgenic animals. Four lines of transgenic mice harboring a human insulin cDNA with expression driven by the goat β-casein gene promoter were generated. The...

Milk samples were from stomachs of 27 nursing cottontail rabbits (Sylvilagus floridanus) within 5 min after cessation of nursing. Four milk samples were directly from mammary glands by hand milking aided by tranquilizer and oxytocin. Means and standard errors for 27 stomach samples for total solids, fat, protein, lactose, and ash were 33.6 +/- .8%, 13.9 +/- 1.7%, 14.6 +/- .5%, 2.22 +/- .05%, an...