background and objective: oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. total antioxidant capacity plays a significant role in the body’s antioxidant defense, so its assessment is a matter of the utmost importance. currently, assessment of this capacity is performed in various s...

The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle i...

The aim of the present work was to estimate the possibility of improving the objectiveness of complement fixation test (CFT) in the diagnosis of dourine by using an ELISA microplate reader. The investigations were carried out on control positive and negative sera and 16 sera obtained for a reference examination. Six modifications of CFT performing and reading methods were compared (tube, microp...

Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette. No commercial kit was available for Bradford microplate assay in Iran. Meanwhile, imported Bradford commercial kits are very expensive and have a long delivery time in Iran. Till now no study or document on Br...

background: bradford protein assay is popular due to its ease of performance and relative sensitivity. many researchers and laboratories in iran use standard assay of bradford by cuvette. no commercial kit was available for bradford microplate assay in iran. meanwhile, imported bradford commercial kits are very expensive and have a long delivery time in iran. till now no study or document on br...

Background and Objective: Oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. Total antioxidant capacity plays a significant role in the body’s antioxidant defense, so its assessment is a matter of the utmost importance. Currently, assessment of this capacity is performed in var...

monplace of all disposable labware. Its most common format consists of 96 individual wells arranged in eight rows and 12 columns with dimensions defined by the American National Standards Institute1 through an initiative sponsored by the Society for Biomolecular Sciences in 1996. There are a myriad of microplate types, including different well densities from several wells to several thousand we...

We describe the ScanMIC method, a colorimetric MIC method for susceptibility testing of gram-negative fermentative bacteria. The method is a slight modification of the National Committee for Clinical Laboratory Standards (NCCLS) recommended broth microdilution method that uses a redox indicator 2,3,5-triphenyltetrazolium chloride (TTC) to enhance the estimate of bacterial growth inhibition in a...

OBJECTIVES Phenol-sulfuric acid reagent is used to measure the concentration of glyco-polymers and -conjugates. There are several uncertainties on glycogen measurement in the tissues. We aimed to improve phenol-sulfuric reagent for microassay of glucose based-glycogen in small tube or microplate. MATERIALS AND METHODS The condition of the reaction was optimized and scaled down for both small ...

background: potency test for control of rubella vaccine is a significant factor to qualify production line and vaccination pro­gram. for this reason, who recommends to use the microtitration method by both vaccine companies and control laborato­ries. then the study was done to improve this test. methods: three rubella virus samples, including an in-house standard, a lot of vaccine and an in- pr...