purpose: the aim of this study was to test the in vitro sensitivity of lymphocytes of patients with head and neck cancer against gamma irradiation and also to find out if the frequencies of chromosomal aberrations correlate with side effects of radiotherapy. patients and methods : peripheral blood of 101 patients with head and neck cancer was collected before the onset of radiotherapy, cultured...

Cytochalasine B is an indicator in the CBMN assay, but can also be geno- or cytotoxic. The aim of study was to determine optimal dose cytochalasine for Cytokinesis-Block Micronucleus (CBMN) assay domestic cat. results revealed a surprisingly high number micronuclei (MN) relation doses used. It found that concentration 2.5 μg/ml enough obtain binuclear cells, with minimal toxic effect on lymphoc...

Background: Tobacco use continues to be prevalent in our society. It contains many substances which are genotoxic in nature. The genotoxicity of these substances can be studied in human peripheral blood lymphocytes by estimating the average number of micronuclei (MN) by cytokinesis block micronuclei assay (CBMN assay). Aims and objectives: To find out whether there is a difference in genotoxici...

the present study investigated the radioprotective efficacy of lentil ( lens culinaris ) sprouts against x-ray radiation-induced cellular damage. lentil seeds were dark germinated at low temperature and the sprout extract was prepared in pbs. free radical scavenging of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl (dpph) assay and then the radioprotective potency of extract (0 to 10...

The present investigation was undertaken to explore the Antimutagenicity activity of Terminalia chebula on micronucleus formation and chromosomal aberration assay in bone marrow cells of Swiss albino mice. The protective effect of Terminalia chebula extract is reported against cyclophosphamide (CP)-induced micronuclei formation and chromosomal aberration in mouse bone marrow cells. The doses na...

The genotoxic effect of endosulfan and the modulatory effect of vitamin C in growing albino rats were studied by bone marrow micronucleus assay. Seven days old male Wistar rats were treated with 3, 6, 9, 12mg/kg Endosulfan orally (10 pups/group),for up to 60 days, at intervals of 24h. For 2 more groups (n=10/group), Endosulfan 9mg/kg and 12mg/kg was administered along with vitamin C (20mg/kg). ...

Objective: Nanotechnology is an emerging technology which has wide applications in many fields. Prime concern of research nanotechnology the synthesis nano-material with controlled size and shape. Recently, biosynthesis metallic nano-particles gained popularity owing to its eco-friendliness. The increasing use Copper nanoparticles (CuNPs) medicine industry demands understanding their potential ...

The multi-endpoint cytokinesis-blocked micronucleus assay is used for assessing chromosome aberrations. We have recently reported that this assay is extremely sensitive to genetic damage caused by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and that the binucleated cells with micronuclei, nucleoplasmic bridges, and nuclear buds in lymphocytes (chromosom...

Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...