To investigate how B. anthracis Sterne spores survive in milk under heat (80 degrees C, 10 min), pasteurization (72 degrees C, 15 s), microfiltration, and pasteurization and microfiltration, the expression levels of genes related to sporulation and germination were tested using real-time PCR assays. Twenty-seven sporulation- and germination-related genes were selected for the target genes. Our ...