dose-dependent pharmacokinetic of phenytoin necessitates the estimation of the maximum rate of metabolism (vm) and the michaelis-menten constant (km) in a concerned population. the aim of this study was to determine the pharmacokinetic parameters of phenytoin in a sample of iranian patients to optimize the antiepileptic pharmacotherapy. fourty patients who received a constant dose of phenytoin ...

The electrocatalytic oxidation of vitamin B6 (pyridoxine hydrochloride) was demonstrated on a Nafion/lead ruthenate pyrochlore modified electrode (designated as NCME) by cyclic voltammetry. The catalytic activity of vitamin B6 was explored in terms of the higher oxidation state of ruthenium species, i.e., Py-Ru/Ru in the pyrochlore network (Py). The mediated mechanism was derived by Michaelis-M...

Nearly 100 years ago Michaelis and Menten published their now classic paper [Michaelis, L., and Menten, M. L. (1913) Die Kinetik der Invertinwirkung. Biochem. Z. 49, 333-369] in which they showed that the rate of an enzyme-catalyzed reaction is proportional to the concentration of the enzyme-substrate complex predicted by the Michaelis-Menten equation. Because the original text was written in G...

in present study, the kinetic parameters of purified trypsin from pyloric caeca of common kilka (c.cultriventris caspia) were measured using the amidolytic (bapna) and esterolytic (tame) substrates. the results of the kinetic comparison between bapna and tame substrates showed that kinetic parameters including maximum velocity (vmax), catalytic constant (kcat) and catalytic efficiency (kcat/km)...

A mathematical model of reaction-diffusion problem with Michaelis-Menten kinetics in catalyst particles arbitrary shape is investigated. Analytical expressions the concentration substrates are derived as functions Thiele modulus, modified Sherwood number, and Michaelis constant. Taylor series approach Akbari-Ganji's method utilized to determine substrate effectiveness factor. The effects factor...

Abstract. Ectoenzymatic activity, prokaryotic heterotrophic abundances and production were determined in the Mediterranean Sea. Sampling was carried out sub-surface, deep chlorophyll maximum layer (DCM), core of Levantine intermediate waters deeper part mesopelagic layers. Michaelis–Menten kinetics assessed using a large range concentrations fluorogenic substrates (0.025 to 50 µM). As consequen...

Ruminally undegraded protein (RUP) values of blood meal (n = 2), hydrolyzed feather meal (n = 2), fish meal (n = 2), meat and bone meal, and soybean meal were estimated using an in situ method, an inhibitor in vitro method, and an inhibitor in vitro technique applying Michaelis-Menten saturation kinetics. Degradation rates for in situ and inhibitor in vitro methods were calculated by regression...

ER aminopeptidase 1 (ERAP1) customizes antigenic peptide precursors for MHC class I presentation and edits the antigenic peptide repertoire. Coding single nucleotide polymorphisms (SNPs) in ERAP1 were recently linked with predisposition to autoimmune disease, suggesting a link between pathogenesis of autoimmunity and ERAP1-mediated Ag processing. To investigate this possibility, we analyzed the...