background: successful embryo transfer (et) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. genetic ...

diethylstilbestrol (des) was widely used in the past, as the morning after contraception, but its application became extremely limited due to several complications including delayed clear cell adenocarcinoma in female infants. however, the use of des increased during the past decade for hormone replacement therapy. the aim of this study was to investigate possible teratogenicity of this synthet...

citrullus colocynthis (cct) is used in traditional medicine to inhibit the implantation of embryos. the objective of this study was to determine the number of embryos per pregnancy and the mortality rate in pregnant mice. 115 vaginal plug-positive mice were divided into 4 groups. the animals were given 30, 60 and 120 mg/kg hydroalcoholic extract of cct until 17th day of gestation. control group...

the aim of this study was to examine the possible morphological and morphometric changes resulting from vitrification of embryos at the cleavage stage. in this study, 30 mice early-cleavage embryos at different stages of cleavage, resulting from in vitro fertilization (ivf) techniques, were examined before and after vitrification. digital images were taken from embryos before and after vitrific...

background: methamphetamine (ma) is one of most common illicit drugs which were reported that nearly half of ma consumers are women. ma can cross through placenta and affects pregnancy and fetus development. objective: our aim was to evaluate effects of injected ma on crown-rump length, head and placental circumference, body weight, histological changes and apoptosis in fetus. materials and met...

background: the basal medium that supports isolated mouse oviduct (imo) is important for supporting embryo development and quality. methods: the culture of ovine ivm/ivf zygotes was done in imo using sofaacibsa and sofaabsa as basal medium of imo and in sofaabsa alone as control. for preparation of imo mature inbred strain c57bl/6 female mice were synchronized and mated with vasectomized males....

abstract background: research studies on reproductive mechanism of laboratory animals are essential for further advancement of assisted reproductive techniques (art). one of these studies includes the assessment of in-vitro development of pre-implantation embryos. the objective was to compare the cleavage rates and morphology of in-vivo formed 2 to 8 cell embryos and blastocysts with in-vitro c...

background: caffeine increases the camp production that stimulates spermatozoa movement. caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization. objective: the aim of this study was to assess the effect of caffeine on sperm's motility, vitality and laboratory fertilization rates in mouse in two t6 and m16 media. m...

background: the luteal phase defect is a common event following the ovarian stimulation. the aim of the present study was to evaluate the use of human chorionic gonadotropine (hcg) and progesterone hormones to improve the luteal phase defect. materials and methods: 60 mice were superovulated routinely with human menopausal gonadotropin (hmg) (7.5u) and hcg (10u). the mice were mated and divided...

introduction: to improve the development of embryo in vitro several attempts have been made, including the application of leukemia inhibitory factor (lif). this factor plays an important role in embryo development and has been shown to enhance in vitro development rate in sheep and mice. there is controversy regarding the effect of lif on the different stages of embryo development. the aim of t...