Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

In this study, three methods are utilized to analyze toxicity produced by methotrexate and the lipid-soluble antifolate, 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine, in human lymphoblasts (WIL-2) and leukemic cells. These methods detect increasingly severe metabolic damage; inhibition of deoxyuridine incorporation into DNA, the reversibility of inhibition of deoxyuridine incorporati...

The physiological function for thiaminase II, a thiamin-degrading enzyme, has eluded investigators for more than 50 years. Here, we demonstrate that this enzyme is involved in the regeneration of the thiamin pyrimidine rather than in thiamin degradation, and we identify a new pathway involved in the salvage of base-degraded forms of thiamin. This pathway is widely distributed among bacteria, ar...

The presence of a hydrogen bond (H-Bond) network has been proved to impact significantly the efficiency organic light-emitting diode (OLED) devices by promoting molecular orientation and structural anisotropy in thin films. design specific compounds control H-Bond formation an amorphous material, hence improve OLED performances, is needed. A successful example given bi-pyridyl-based family n-ty...

Novel synthetic approach to mono-O-protected anti-conformationally constrained pyrimidine acyclic nucleoside was attained from the coupling of lithiated 2,4-dimethoxy-6-methylpyrimidine with 1-benzyloxy-3-(tert-butyldiphenylsilyloxy)propan-2-one, followed by the sequential reactions of methylthiomethylation, cyclization, hydroxylation, and dealkylation.

We report a one-pot, two-step strategy to synthesize fluorescent gold nanodots (AuNDs) co-modified with 1-(10-mercaptodecyl)-5-methylpyrimidine-2,4-dione (TSH) and 11-mercaptoundecanoic acid (MUA) through a ligand exchange reaction and demonstrate their capability of selective sulfide sensing in aqueous media on the basis of fluorescence recovery.