Twenty one bacterial isolates were isolated from methyl parathion treated agricultural soil from in and around the Gwalior city, Madhya Pradesh to study the degradation of Methyl Parathion. Isolation was done by enrichment in Basal mineral medium with 50 mg/L methyl parathion as sole carbon and energy source. No supplement as a carbon and phosphorus source was added in medium. Methyl parathion ...

Sublethal doses of methyl parathion (O,O-dimethyl-O-nitrophenyl- thiophosphate) injected intraperitoneally to 15 and 21 day old rat pups induced regional alterations in the central nervous system (CNS) in the levels of total RNA, total proteins, modulatory protein Calmodulin (CaM), in the activity levels of membrane bound enzyme Ca(2+)-ATPase and phospholipids. Levels of RNA and total proteins ...

Three species of freshwater Brazilian fishes (pacu, Piaractus mesopotamicus; piavussu, Leporinus macrocephalus, and curimbatá, Prochilodus lineatus) were exposed to an acute dose of 5 ppm methyl parathion organophosphate pesticide. Three to five individuals per species were exposed, one at a time, to 40 liters tap water spiked with Folidol 600. Pesticide concentrations and cholinesterase (ChE) ...

An optical microbial biosensor was described for the detection of methyl parathion pesticide. Whole cells of Flavobacterium sp. were immobilized by trapping in glass fiber filter and were used as biocomponent along with optic fiber system. Flavobacterium sp. has the organophosphorus hydrolase enzyme, which hydrolyzes the methyl parathion into detectable product p-nitrophenol. The immobilized mi...

The pharmacokinetics and placental transfer of a single dermal 10.0 mg (10microCi)/kg dose of uniformly phenyl-labeled [14C] methyl parathion (0,0-dimethyl 0-4-nitrophenyl phosphorothioate) were investigated in pregnant Sprague-Dawley rats at 14-18 days of gestation. Three rats were killed at each time interval: 1, 2, 4, 12, 24, 48, 72, and 96 h after dosing. Radioactivity disappeared biexponen...

Pesticides are indispensable in today’s agriculture. Methyl-parathion hydrolase (MPH, E.C.3.1.8.1) could hydrolyze organophosphorus pesticides, including methyl-parathion. MPH rehabilitate soil and water resources contaminated by pesticides. However, natural MPHs generally exhibited a low tolerance to high temperatures catalytic efficiency. In this study, we improved the efficiency toward methy...

rodlet cells are frequently found in teleost fishes and although their role in organisms is not completely understood. the occurrence of these cells are related to stress and may undergo changes in contaminated environments, thereby allowing their use as biomarkers. this hypothesis is tested in the present study. thirty specimens of oreochromis niloticus were divided into three groups, two grou...

In the present study, organophosphorus pesticides (OPs) (diazinon, methyl parathion, and parathion) were oxidized by bubbling ozone into a glass reactor. OP residues were detected using HPLC and ozonation intermediates were identified using GC-MS. The degradation of OPs followed pseudo-first-order kinetics through direct ozone oxidation and indirect hydroxyl radical oxidation. Diazinon, based o...

In this work, we report a green method for the synthesis of gold nanoparticles (GNP) using the aqueous extract of red tomato (Lycopersicon esculentum). We believe that citric acid and ascorbic acid present in tomato juice are responsible for the reduction of gold ions. This biosynthesized GNP in the presence of sodium dodecyl sulfate has been used as a colorimetric sensor to detect and estimate...

Methyl parathion induced alterations in the level of monoamines, viz. norepinephrine, dopamine and serotonin were studied in discrete regions of developing central nervous system of rat pups. A significant decrease in the level of monoamines noticed in methyl parathion toxicosis may be related to the altered neuronal activity and inefficiency, leading to depression and impairment in various beh...