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The cation of methyl green carries two positive charges, that of malachite green only one; but the two dyes behave towards tissue-constituents in almost exactly the same way. These dyes are not specific for chromatin. They colour certain objects that are devoid of DNA, even when they are used in very dilute solution. The granules of cells called Kornchenzellen in the connective tissue of the co...

There exist many methods for the application of the Gram stain to histological sections, and with all of them there is generally no difficulty in recognising Gram-positive organisms. However, with most of the well-recognised methods Gram-negative bacteria are difficult to distinguish from the background. Twort's method distinguishes Gram-negative bacteria from the background but is relatively t...

We show that the spectral phasor approach of the fluorescent dye Pyronin Y (PY) can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. We applied spectral phasors to isolate intracellular RNA species with similar spectral properties. We identified at least 4 different PY labeled species in live cells and further spatially mapped their presence at the pi...

A new histochemical technique for morphological studies of mononuclear cells and granulocytes, based on fluorescent staining with methyl green, pyronine Y, and stilbene-isothiocyanato disulfonic acid (MPS stain) is described. The method was applied to mononuclear cells isolated from the blood of normal human subjects by Ficoll-Isopaque density centrifugation. Three cell populations were disting...

The distribution and renewal of RNA in mature rod visual cells has been studied by histochemical and autoradiographic technique in the rat and the frog. Staining with methyl green and pyronin, combined with ribonuclease extraction, revealed the presence of RNA in the nucleus and inner segments of rods in both species. Synthesis of RNA was studied by injecting cytidine-H, a radioactive precursor...

The presence of effusion in association with multiple myeloma or macroglobulinemia is an unusual finding which generally occurs late in the course of the disease. Occasionally, cytoligic detection is a diagnostic problem. In both conditions, the fluid is characrerized by a high specific gravity and high protein content. In myeloma, plasma cells at varying stages of differentiation are present, ...

Lymph nodes and splenic tissue from patients with congenital agammaglobulinemia and dysgammaglobulinemia and from normal subjects were studied with the use of immunofluorescence and histochemical stains to determine the site of synthesis of the 19S gamma(1)-globulins. The two patients with dysgammaglobulinemia had high serum concentrations of the 19S gamma(1)-globulins and a marked deficit of t...

A high proliferative activity of the odontogenic epithelium in ameloblastoma (AM) and keratocystic odontogenic tumor (KOT) has been demonstrated. However, no previous study has simultaneously evaluated cell proliferation and apoptotic indexes in AM and KOT, comparing both lesions. The aim of this study was to assess and compare cell proliferation and apoptotic rates between these two tumors. Sp...

1. Basic dyes (methyl green/pyronin, and toluidin blue), in conjunction with ribonuclease, may be used to detect the cytochemical localization of ribonucleic acid. This technique has been examined critically. 2. The fixative used is important and results vary with different materials. It is suggested that Zenker and Serra fixation should be compared in preliminary investigations. Unexplained an...